中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2013年
2期
128-132
,共5页
李玉军%于文娟%于层%孙显路
李玉軍%于文娟%于層%孫顯路
리옥군%우문연%우층%손현로
胰腺肿瘤%受体,肾上腺素能β2%多态性,单核苷酸
胰腺腫瘤%受體,腎上腺素能β2%多態性,單覈苷痠
이선종류%수체,신상선소능β2%다태성,단핵감산
Pancreatic neoplasms%Receptors,adrenergic,beta-2%Polymorphism,single nucleotide
目的 探讨β2肾上腺素能受体(β2-AR)基因16、27位密码子单核苷酸多态性(SNPs)与胰腺癌临床病理特征、β2-AR蛋白表达情况的相关性.方法 应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法结合DNA测序法检测64例胰腺癌组织和20例癌旁胰腺组织中β2-AR 16、27位点的多态性.免疫组织化学技术检测肿瘤组织中β2-AR蛋白的表达情况.结果 16、27位点的基因型多态性分布均符合Hardy-Weinberg平衡定律.16、27位点的基因型和等位基因分布频率在胰腺癌组和癌旁胰腺组间差异均无统计学意义(P＞0.05).16位点基因型分布频率与患者的性别、年龄、肿瘤分化程度、肿瘤大小无相关性(P＞0.05),与淋巴结转移、TNM分期、1年生存率及β2-AR蛋白的表达水平相关(P=0.03、0.05、0.04).G等位基因与淋巴结转移、高TNM分期、低1年生存率及β2-AR蛋白的高表达相关(P=0.01、0.03、0.02、0.02).27位点基因型分布频率与患者的性别、年龄、肿瘤分化程度、肿瘤大小、淋巴结转移、TNM分期、1年生存期及β2-AR蛋白的表达水平均无相关性(P＞0.05).结论 β2-AR基因16位点SNPs与胰腺癌的生物学行为相关.G等位基因提示有淋巴结转移可能、TNM分期高、1年生存率低及β2-AR蛋白高表达.它可能通过增加β2-AR蛋白的表达促进胰腺癌进展与转移.检测β2-AR基因16位点SNPs有助于评估患者胰腺癌的生物学行为和预后,有望成为胰腺癌基因治疗的新靶点.
目的 探討β2腎上腺素能受體(β2-AR)基因16、27位密碼子單覈苷痠多態性(SNPs)與胰腺癌臨床病理特徵、β2-AR蛋白錶達情況的相關性.方法 應用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)方法結閤DNA測序法檢測64例胰腺癌組織和20例癌徬胰腺組織中β2-AR 16、27位點的多態性.免疫組織化學技術檢測腫瘤組織中β2-AR蛋白的錶達情況.結果 16、27位點的基因型多態性分佈均符閤Hardy-Weinberg平衡定律.16、27位點的基因型和等位基因分佈頻率在胰腺癌組和癌徬胰腺組間差異均無統計學意義(P＞0.05).16位點基因型分佈頻率與患者的性彆、年齡、腫瘤分化程度、腫瘤大小無相關性(P＞0.05),與淋巴結轉移、TNM分期、1年生存率及β2-AR蛋白的錶達水平相關(P=0.03、0.05、0.04).G等位基因與淋巴結轉移、高TNM分期、低1年生存率及β2-AR蛋白的高錶達相關(P=0.01、0.03、0.02、0.02).27位點基因型分佈頻率與患者的性彆、年齡、腫瘤分化程度、腫瘤大小、淋巴結轉移、TNM分期、1年生存期及β2-AR蛋白的錶達水平均無相關性(P＞0.05).結論 β2-AR基因16位點SNPs與胰腺癌的生物學行為相關.G等位基因提示有淋巴結轉移可能、TNM分期高、1年生存率低及β2-AR蛋白高錶達.它可能通過增加β2-AR蛋白的錶達促進胰腺癌進展與轉移.檢測β2-AR基因16位點SNPs有助于評估患者胰腺癌的生物學行為和預後,有望成為胰腺癌基因治療的新靶點.
목적 탐토β2신상선소능수체(β2-AR)기인16、27위밀마자단핵감산다태성(SNPs)여이선암림상병리특정、β2-AR단백표체정황적상관성.방법 응용취합매련반응-한제성편단장도다태성(PCR-RFLP)방법결합DNA측서법검측64례이선암조직화20례암방이선조직중β2-AR 16、27위점적다태성.면역조직화학기술검측종류조직중β2-AR단백적표체정황.결과 16、27위점적기인형다태성분포균부합Hardy-Weinberg평형정률.16、27위점적기인형화등위기인분포빈솔재이선암조화암방이선조간차이균무통계학의의(P＞0.05).16위점기인형분포빈솔여환자적성별、년령、종류분화정도、종류대소무상관성(P＞0.05),여림파결전이、TNM분기、1년생존솔급β2-AR단백적표체수평상관(P=0.03、0.05、0.04).G등위기인여림파결전이、고TNM분기、저1년생존솔급β2-AR단백적고표체상관(P=0.01、0.03、0.02、0.02).27위점기인형분포빈솔여환자적성별、년령、종류분화정도、종류대소、림파결전이、TNM분기、1년생존기급β2-AR단백적표체수평균무상관성(P＞0.05).결론 β2-AR기인16위점SNPs여이선암적생물학행위상관.G등위기인제시유림파결전이가능、TNM분기고、1년생존솔저급β2-AR단백고표체.타가능통과증가β2-AR단백적표체촉진이선암진전여전이.검측β2-AR기인16위점SNPs유조우평고환자이선암적생물학행위화예후,유망성위이선암기인치료적신파점.
Objective To investigate the single nucleotide polymorphisms (SNPs) at codons 16 and 27 of β2-AR gene in pancreatic carcinoma and non-neoplastic pancreatic tissues,and the correlations between these SNPs and the expression of β2-AR protein in pancreatic carcinoma.Methods A total of 64 cases of pancreatic carcinoma (PC) and 20 non-neoplastic pancreatic tissues (NPC) were genotyped at codons 16 and 27 by PCR-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing.The correlations between the distribution of genotypes and clinicopathological characteristics of pancreatic carcinoma were analyzed.The expression of β2-AR protein was detected by immunohistochemistry in pancreatic carcinoma.Results The distributions of genotype frequency at codons 16 and 27 in PC and NPC were in accordance with the Hardy-Weinbery equeilibrium.The frequencies of their genotypes (AA,AG and GG) and frequencies of alleles A and G at codon 16 between PC and NPC showed no difference.The genotype frequencies were associated with TNM grade,lymph node metastasis,one-year survival rate (P=0.03,0.05,0.04),but they were not associated with patients' gender,age,histological differentiation and size of tumor.The allele G at codon 16 was frequently appeared in tumors with high TNM grade,lymph node metastasis,low one-year survival rate (P＝ 0.01,0.03,0.02),and high expressions of β2-AR protein (P =0.02).The frequencies of two genotypes (CC and CG) and frequencies of alleles C and G at codon 27 showed no difference between PC and NPC.The genotype frequencies and allele frequencies of codon 27 were not associated with patients' clinicopathological features,and expressions of β2-AR protein.Conclusions SNPs of β2-AR gene were associated with biological behaviors of pancreatic carcinoma.Allele G at codon 16 was associated with high risks of lymph node metastasis,high TNM grade,low one-year survival rate,and high expressions of β2-AR protein.Allele G at codon 16 might facilitate the progression and metastasis of pancreatic carcinoma through elevating the expression of β2-AR.SNPs at codon 16 of β2-AR are new useful biomarkers for predicting biological behaviors and survival of pancreatic carcinoma and might be used as a new gene therapeutic target.
