CAJ | 학술논문

目的：探讨Nucleostemin基因及PCNA在非小细胞肺癌（NSCLC）组织中的检测及意义。方法：采用免疫组织化学SP法对53例NSCLC患者的非小细胞肺癌组织和15例癌旁正常者的组织中NS蛋白进行检测，并对其与NSCLC患者临床病理特征关系进行分析。结果：NS蛋白在NSCLC中的表达率为54.7%（29/53）明显高于癌旁正常组织中的表达率0（0/15），NS蛋白腺癌组织的表达率为65.2%（15/23）明显高于鳞癌组织的表达率46.7%（14/30），高、中分化组织的表达率42.9%（15/35）明显低于低分化组织的表达率77.8%（14/18），比较差异均有统计学意义（P<0.05），与患者TNM分期及淋巴结转移无关（P>0.05）。NSCLC组织中PCNA阳性率71.7%（38/53）明显高于癌旁正常组织6.7%（1/15），差异有统计学意义（P<0.05）。结论：NSCLC患者非小细胞肺癌组织中，NS基因mRNA和蛋白存在明显的高表达趋势，PCNA阳性率显著增高，有利于肿瘤细胞恶性增殖，因而是一种临床应用价值较高的肿瘤分子标志物。
목적：탐토Nucleostemin기인급PCNA재비소세포폐암（NSCLC）조직중적검측급의의。방법：채용면역조직화학SP법대53례NSCLC환자적비소세포폐암조직화15례암방정상자적조직중NS단백진행검측，병대기여NSCLC환자림상병리특정관계진행분석。결과：NS단백재NSCLC중적표체솔위54.7%（29/53）명현고우암방정상조직중적표체솔0（0/15），NS단백선암조직적표체솔위65.2%（15/23）명현고우린암조직적표체솔46.7%（14/30），고、중분화조직적표체솔42.9%（15/35）명현저우저분화조직적표체솔77.8%（14/18），비교차이균유통계학의의（P<0.05），여환자TNM분기급림파결전이무관（P>0.05）。NSCLC조직중PCNA양성솔71.7%（38/53）명현고우암방정상조직6.7%（1/15），차이유통계학의의（P<0.05）。결론：NSCLC환자비소세포폐암조직중，NS기인mRNA화단백존재명현적고표체추세，PCNA양성솔현저증고，유리우종류세포악성증식，인이시일충림상응용개치교고적종류분자표지물。
Objective:To investigate the detection and significance of Nucleostemin proteins and PCNA in non-small cell lung cancer(NSCLC) tissue.Method:The clinical studies were detected by immunohistochemistry SP method in 53 cases of NSCLC patients with non-small cell lung cancer and 15 cases of adjacent normal tissues NS protein and its clinical and pathological characteristics of patients with NSCLC were analyzed.Result:The NS protein expression in NSCLC was 54.7%(29/53),it was significantly higher than in adjacent normal tissue expression rate(0/15);Adenocarcinoma of the NS protein expression was 65.2%(15/23),it was significantly higher than the expression rate 46.7%(14/30)of the squamous cell carcinoma;The expression of differentiated tissues was 42.9%(15/35),it was significantly lower than the rate of poorly differentiated tissues 77.8%(14/18),there were statistically significant differences(P<0.05).And it had no relationship with TNM stages and lymph node metastasis(P>0.05).The positive rate of PCNA in NSCLC group was 71.7%(38/53),it was significantly higher than that in normal tissues6.7%(1/15),the difference was statistically significant between the groups(P<0.05).Conclusion:NSCLC patients with non-small cell lung cancer,NS mRNA and protein high expression of trend is conducive to the malignant proliferation of tumor cells,the positive rate of PCNA is significantly higher,and thus is a high clinical value of tumor molecular markers.