中国中医药现代远程教育
中國中醫藥現代遠程教育
중국중의약현대원정교육
CHINESE MEDICINE MODERN DISTANCE EDUCATION OF CHINA
2012年
24期
143-145
,共3页
SH-SY5Y 细胞%四氢吡啶离子%干姜%大黄%丹参%热性%寒性%细胞凋亡
SH-SY5Y 細胞%四氫吡啶離子%榦薑%大黃%丹參%熱性%寒性%細胞凋亡
SH-SY5Y 세포%사경필정리자%간강%대황%단삼%열성%한성%세포조망
SH-SY5Y cells%MPP+%Zingiber officinale%Rheum palmatum%Salvia miltiorrhiza%Hot%Cold%Apoptosis
目的研究干姜、大黄和丹参水提物对四氢吡啶离子(MPP+)诱导 SH-SY5Y 细胞凋亡的影响.方法体外培养 SH-SY5Y 细胞,建立 MPP+致 SH-SY5Y 细胞凋亡的模型,同时给予干姜、大黄和丹参水提物干预.应用 MTT 比色法检测细胞活力,流式细胞仪检测细胞凋亡率.结果0.5 mmol?L-1 MPP+作用于 SH-SY5Y 细胞60 h,MTT 值降低到(30.43±2.69)%(P<0.01 vs. control),Hoechst 33258染色可见明显的颗粒状和固缩状荧光,凋亡率增加到(40.52±1.68)%(P<0.01 vs. control),而干姜水提物能明显改善以上的凋亡特性,显著升高其 MTT 值,降低凋亡率(P<0.05 vs. model group),大黄和丹参水提物则对 MPP+诱导的 SH-SY5Y 细胞凋亡无保护作用.结论干姜对 MPP+诱导的 SH-SY5Y 细胞凋亡有显著保护作用,而大黄和丹参却无此保护作用,中药药性不同,其抗凋亡作用亦不同.
目的研究榦薑、大黃和丹參水提物對四氫吡啶離子(MPP+)誘導 SH-SY5Y 細胞凋亡的影響.方法體外培養 SH-SY5Y 細胞,建立 MPP+緻 SH-SY5Y 細胞凋亡的模型,同時給予榦薑、大黃和丹參水提物榦預.應用 MTT 比色法檢測細胞活力,流式細胞儀檢測細胞凋亡率.結果0.5 mmol?L-1 MPP+作用于 SH-SY5Y 細胞60 h,MTT 值降低到(30.43±2.69)%(P<0.01 vs. control),Hoechst 33258染色可見明顯的顆粒狀和固縮狀熒光,凋亡率增加到(40.52±1.68)%(P<0.01 vs. control),而榦薑水提物能明顯改善以上的凋亡特性,顯著升高其 MTT 值,降低凋亡率(P<0.05 vs. model group),大黃和丹參水提物則對 MPP+誘導的 SH-SY5Y 細胞凋亡無保護作用.結論榦薑對 MPP+誘導的 SH-SY5Y 細胞凋亡有顯著保護作用,而大黃和丹參卻無此保護作用,中藥藥性不同,其抗凋亡作用亦不同.
목적연구간강、대황화단삼수제물대사경필정리자(MPP+)유도 SH-SY5Y 세포조망적영향.방법체외배양 SH-SY5Y 세포,건립 MPP+치 SH-SY5Y 세포조망적모형,동시급여간강、대황화단삼수제물간예.응용 MTT 비색법검측세포활력,류식세포의검측세포조망솔.결과0.5 mmol?L-1 MPP+작용우 SH-SY5Y 세포60 h,MTT 치강저도(30.43±2.69)%(P<0.01 vs. control),Hoechst 33258염색가견명현적과립상화고축상형광,조망솔증가도(40.52±1.68)%(P<0.01 vs. control),이간강수제물능명현개선이상적조망특성,현저승고기 MTT 치,강저조망솔(P<0.05 vs. model group),대황화단삼수제물칙대 MPP+유도적 SH-SY5Y 세포조망무보호작용.결론간강대 MPP+유도적 SH-SY5Y 세포조망유현저보호작용,이대황화단삼각무차보호작용,중약약성불동,기항조망작용역불동.
Objective To investigate the protective effects of water extracts of Zingiber officinale, Rheum palmatum and Salvia miltiorrhiza on SH-SY5Y cells apoptosis induced by MPP+ in culture. Methods SH-SY5Y cells were cultured in vitro and MPP+ induced SH-SY5Y cells apoptotic model was established and treated with Water extracts of Z. officinale, R. palmatum and S. miltiorrhiza. The cell Viability was measured by MTT test, the morphology of SH-SY5Y cells was observed by Hoechst33258 staining and the percentage of apoptosis by FCM. Results The cell activity decreased remarkably to (30.43±2.69) % (P<0.01 vs. control) induced by 0.5 mmol/L MPP+ for 60 h and were positively stained with fluorochrome Hoechst 33258, presenting the percentage of apoptosis as (40.52±1.68) % (P<0.01 vs. control), However, water extracts of Z. officinale obviously improved MTT value, reduced the percentage of apoptosis (P<0.05 vs. model group), and water extracts of R. palmatum and S. miltiorrhiza. almost had no effect on the apoptosis of SH-SY5Y cells. Conclusion Z. officinale has markedly protective effects on SH-SY5Y cells apoptosis induced by MPP+, but R. palmatum and S. miltiorrhiza. show no protective effects, as the different natures of TCM, the anti-apoptotic effects of TCM are different neither.
