CAJ | 학술논문

对枯草芽孢杆菌菌株Tpb55的安全性进行了合理的评价，就菌粉Tpb55对试验动物进行了相关的毒理学试验。结果表明，菌株Tpb55对小鼠、大鼠急性经口LD50大于5000 mg·kg?1，对大鼠急性经皮LD50大于5000 mg·kg?1；对兔皮肤刺激强度为无刺激性，眼刺激平均指数48 h后为0，眼刺激强度为无刺激性；对豚鼠致敏率为0，属 I 级弱致敏物，无致敏性；对小鼠骨髓嗜多染红细孢微核以及性染色体及常染色体单价体等3项致突变试验为阴性，说明菌株Tpb55没有致突变作用。
대고초아포간균균주Tpb55적안전성진행료합리적평개，취균분Tpb55대시험동물진행료상관적독이학시험。결과표명，균주Tpb55대소서、대서급성경구LD50대우5000 mg·kg?1，대대서급성경피LD50대우5000 mg·kg?1；대토피부자격강도위무자격성，안자격평균지수48 h후위0，안자격강도위무자격성；대돈서치민솔위0，속 I 급약치민물，무치민성；대소서골수기다염홍세포미핵이급성염색체급상염색체단개체등3항치돌변시험위음성，설명균주Tpb55몰유치돌변작용。
The toxicological safety of Bacillus subtilis strain Tpb55 was evaluated. The results showed that the acute oral and cute dermal toxicity of LD50 of Tpb55 was greater than 5000 mg·kg?1, respectively. No irritation to rabbit skin and eye was found, and the rate of dermal sensitization was 0 after 48 h. Thus, it can be concluded that the acute toxicity of Tpb55 both through mouth and skin was in lower level with no irritations to rabbit skin and eye detected. Its dermal hypersusceptibility rate was zero for the guinea pigs, being a weak sensitized tar of gradeⅠ, there were no significant differences between the experimental groups and the control group in micronucleus assay and sperm shape abnormality. Therefore, the strain Tpb55 is a safe biological bacterium with low toxicity, no irritation, no sensitization and no mutagenic effect.