CAJ | 학술논문

地衣芽孢杆菌Bacillus licheniformis W10是1株生防细菌，其能产生抗菌蛋白，对多种植物病原菌有较好的抑制效果。本试验研究了菌株W10产生抗菌蛋白的发酵条件，为工厂化生产工艺提供技术支持。试验结果表明，接种菌龄12 h，2%接种量，发酵液pH 7.0，发酵培养60 h是菌株W10产生抗菌蛋白的适宜发酵条件。控制发酵温度有利于抗菌蛋白积累，第一段温度为30℃培养20 h，第二段温度为28℃至发酵结束。最佳溶氧量为20%。2次补料发酵效果要高于分批发酵，补料为500 mL含10 g·L?1葡萄糖和5 g·L?1蛋白胨的培养基；选用200 mg·L?1聚氧丙烯聚氧乙烯甘油醚（GPE消泡剂，泡敌）为消泡剂。利用优化的控制条件发酵培养菌株W10，与优化前相比，对目标病菌的抑菌率增加了34.6%。
지의아포간균Bacillus licheniformis W10시1주생방세균，기능산생항균단백，대다충식물병원균유교호적억제효과。본시험연구료균주W10산생항균단백적발효조건，위공엄화생산공예제공기술지지。시험결과표명，접충균령12 h，2%접충량，발효액pH 7.0，발효배양60 h시균주W10산생항균단백적괄의발효조건。공제발효온도유리우항균단백적루，제일단온도위30℃배양20 h，제이단온도위28℃지발효결속。최가용양량위20%。2차보료발효효과요고우분비발효，보료위500 mL함10 g·L?1포도당화5 g·L?1단백동적배양기；선용200 mg·L?1취양병희취양을희감유미（GPE소포제，포활）위소포제。이용우화적공제조건발효배양균주W10，여우화전상비，대목표병균적억균솔증가료34.6%。
Bacillus licheniformis W10 is an antagonistic bacterium, which can produce antifungal protein inhibiting growth to a variety of plant pathogenic fungi. The technical conditions of fermentation W10 to produce antifungal protein were studied. The results indicated that yield of antifungal protein was promising under the conditions of inoculation bacteria for 12 h, 2%inoculum volume, fermentation for 60 h and pH 7.0. During the fermentation, changing culture temperature was favorable to the antifungal protein accumulation and increased its inhibitory effect against plant pathogen Botrytis cinerea, with fermentation temperature of first stage was 30℃ for 20 h, and then was adjusted to 28℃until the end of fermentation. The suitable dissolved oxygen value was 20%. The better fermentation method was two fed-batch culture with 10 g·L?1 glucose and 5 g·L?1 peptone as the supplement medium. The defoaming substance was defoamer GPE (polyoxypropylene-polyoxyethylene glycerol ether) with 200 mg·L?1. Under the optimized fermentation conditions, the inhibitory effect of the strain W10 against B. cinerea reached 99.5%, increasing 34.6%compared with the control.