CAJ | 학술논문

目的：应用多位点可变数目串联重复序列分型（ multiple locus variable number tandem repeat analysis ， MLVA）技术，初步探讨广东地区羊种3型布鲁氏菌的基因多态性特征分布。方法采用MLVA-16分型技术对广东地区患者分离的羊种3型布鲁氏菌株进行PCR 扩增、琼脂糖凝胶电泳和毛细管电泳等技术，并应用Bio Numerics（Version 5．0）软件进行布鲁氏菌基因多态性研究。结果43株布鲁氏菌菌株间的MLVA分型相似值介于68．2％～100％，在100％的水平上分成32种基因型别，其中27株（62．8％）为单一基因型，另外16株（37．2％）分属于其他5种基因型，各基因型分别含2～5株菌。结论广东地区羊种3型布鲁氏菌MLVA基因型呈现多态性分布特征，没有出现优势基因型别，单一特有基因型占了62．8％。
목적：응용다위점가변수목천련중복서렬분형（ multiple locus variable number tandem repeat analysis ， MLVA）기술，초보탐토엄동지구양충3형포로씨균적기인다태성특정분포。방법채용MLVA-16분형기술대엄동지구환자분리적양충3형포로씨균주진행PCR 확증、경지당응효전영화모세관전영등기술，병응용Bio Numerics（Version 5．0）연건진행포로씨균기인다태성연구。결과43주포로씨균균주간적MLVA분형상사치개우68．2％～100％，재100％적수평상분성32충기인형별，기중27주（62．8％）위단일기인형，령외16주（37．2％）분속우기타5충기인형，각기인형분별함2～5주균。결론엄동지구양충3형포로씨균MLVA기인형정현다태성분포특정，몰유출현우세기인형별，단일특유기인형점료62．8％。
Objective To investigate the polymorphism of Brucella melitensis biovar 3 ( B.melitensis biovar 3) strains isolated from Guangdong province .Methods PCR assays followed by agar-ose gel electrophoresis and capillary electrophoresis based on the multiple locus variable number of tandem repeats (VNTR) analysis (MLVA) were performed to analyze 43 clinic isolates of B.melitensis biovar 3 strains isolated from clinical patients in Guangdong province .Results MLVA typing showed that the simi-larities of the analyzed locus among 43 strains of Brucella ranged from 68.2%to 100%.32 genotypes identi-fied among the isolates were identical (100%similarity).27 out of 43 strains (62.8%) were single geno-types, while the other 16 strains (37.2%) belonged to 5 other genotypes with 2 to 5 strains in each of them . Conclusion B.melitensis biovar 3 isolates showed polymorphism distribution in Guangdong province as in-dicated by MLVA typing analysis .Single-genotype isolates accounted for 62.8% of all studied strains.No predominant genotype was found among all isolates .