CAJ | 학술논문

目的：构建对奥沙利铂（L-OHP）耐药的人结肠癌细胞系SW480/L-OHP ，并初步探索其耐药特性。方法以逐渐递增L-OHP浓度方法构建人结肠癌耐药细胞系模型SW480/L-OHP ，观察其生长规律和绘制生长曲线；M TT法检测其耐药性和计算耐药指数；流式细胞仪检测其细胞周期和罗丹明123蓄积量。结果成功构建稳定耐药的SW480/L-OHP ，耐药指数为（6．23±2．33），且对其他抗肿瘤药也有一定程度的交叉耐药。体外细胞群体倍增时间延长；细胞内罗丹明累计实验测定其含量明显减少，细胞周期显示其出现G2/M 期阻滞。结论SW480/L-OHP细胞株耐药性较稳定，具备耐药细胞的基本生物学特征，其耐药机制可能与细胞周期改变和P-gp功能增强有关。
목적：구건대오사리박（L-OHP）내약적인결장암세포계SW480/L-OHP ，병초보탐색기내약특성。방법이축점체증L-OHP농도방법구건인결장암내약세포계모형SW480/L-OHP ，관찰기생장규률화회제생장곡선；M TT법검측기내약성화계산내약지수；류식세포의검측기세포주기화라단명123축적량。결과성공구건은정내약적SW480/L-OHP ，내약지수위（6．23±2．33），차대기타항종류약야유일정정도적교차내약。체외세포군체배증시간연장；세포내라단명루계실험측정기함량명현감소，세포주기현시기출현G2/M 기조체。결론SW480/L-OHP세포주내약성교은정，구비내약세포적기본생물학특정，기내약궤제가능여세포주기개변화P-gp공능증강유관。
Objective To establish oxaliplatin (L-OHP)-resistant human colon carcinoma cell line (SW480/L-OHP) and to preliminarily study its resistant characteristics . Methods Resistance to L-OHP was induced by continuously exposing the human colon carcinoma SW 480 cells to gradually increasing con-centrations of L-OHP . The rate of cell growth was measured and the grow th curve plotted . The extent of resistance to L-OHP was determined by MTT assay and the resistant index calculated . Cell cycle phase distribution and intracellular Rhodamine 123 accumulation were assessed by flow cytometry . Results The SW480/L-OHP cells with stable resistance were successfully established with the resistance index of (6 .23 ± 2 .33) . SW480/L-OHP cells were cross-resistant to other chemotherapeutic drugs to some extent . In vitro doubling time of the cell population was increased . Intracellular accumulation of rhodamine was significantly reduced . Cell cycle arrested at G2/M phase . Conclusions SW480/L-OHP cell line demonstrates relatively stable resistant phenotypes with essential characteristics of multi-drug re-sistance . The underlying mechanisms may be related to the disturbance of cell cycle progression and the increase of P-glycoprotein function .