CAJ | 학술논문

以云烟97、云烟87、Beinhart1000-1等11个烟草品种及N. glutinosa、N. plumbaginifolia为材料，采用SCoT分子标记技术，对上述11个烟草品种、3个烟草种间的遗传关系进行分析，并采用SCoT分子标记对10株N. plumbaginifolia和云烟87的杂交后代进行杂种鉴定。结果显示，对于云烟97，36条SCoT引物均扩增出了清晰的条带；分析11个烟草品种的PCR扩增条带，可筛选出的9条SCoT引物，共扩增出条带118条，其中多态性条带42条，多态性比例35.59%，各烟草品种间的相似系数均高于0.9；对2个野生种和普通烟草种进行扩增，筛选出的9条引物共扩增出163条带，其中多态性条带135条，多态性比例为82.82%，其中SP27号引物的多态性比例最高，达到了90.91%。10株杂交后代均可见父、母本特异条带，部分出现父母本不具有的特异条带。结果表明，SCoT引物不适于对普通烟草种内不同类型的遗传分析，可用于烟草种间的遗传关系的分析，也可用于远缘杂种的鉴定。
이운연97、운연87、Beinhart1000-1등11개연초품충급N. glutinosa、N. plumbaginifolia위재료，채용SCoT분자표기기술，대상술11개연초품충、3개연초충간적유전관계진행분석，병채용SCoT분자표기대10주N. plumbaginifolia화운연87적잡교후대진행잡충감정。결과현시，대우운연97，36조SCoT인물균확증출료청석적조대；분석11개연초품충적PCR확증조대，가사선출적9조SCoT인물，공확증출조대118조，기중다태성조대42조，다태성비례35.59%，각연초품충간적상사계수균고우0.9；대2개야생충화보통연초충진행확증，사선출적9조인물공확증출163조대，기중다태성조대135조，다태성비례위82.82%，기중SP27호인물적다태성비례최고，체도료90.91%。10주잡교후대균가견부、모본특이조대，부분출현부모본불구유적특이조대。결과표명，SCoT인물불괄우대보통연초충내불동류형적유전분석，가용우연초충간적유전관계적분석，야가용우원연잡충적감정。
Genetic relationship of 11 tobacco varieties, e.g. Yunyan97, Yunyan87, Beinhart1000-1, and three interspecific tobacco varieties were analyzed using SCoT molecular marker technology. Ten hybrid offsprings of N. plumbaginifolia and Yunyan87 were identified. Results show that all 36 primers of the SCoT were amplified clear bands with Yunyan97 as template. Nine SCoT primers were selected from 11 tobacco varieties of PCR amplification, by which 118 bands were produced. Of them 42 polymorphic bands were found with polymorphism ratio of 35.59%. Similarity coefficients of all tobacco varieties were higher than 0.9. As for two wild species and an ordinary tobacco species, nine SCoT primers were selected from amplification results, by which 163 bands were produced, of them 135 polymorphic bands were found with polymorphism ratio of 82.82%. Primer SP27 showed the highest polymorphism of 90.91%. All 10 hybrid offsprings showed specific bands of male and female parent, while some of them showed new bands different from their parents. The above results indicated that existing SCoT primers were suitable for identifying genetic relationship between species and distant hybrids, but not suitable for varieties within species.