CAJ | 학술논문

为明确微生物制剂MP提高烟叶品质的原因，以该制剂的代谢产物作为研究材料，采用液相色谱串联质谱法（LC-MS/MS）分析了微生物制剂MP代谢产物的蛋白质组成分。结果共鉴定出35个非重复蛋白质，蛋白等电点几乎均在4-7范围内，分子量均大于20 kD。KEGG代谢通路分析显示，所鉴定的蛋白几乎都与代谢有关，其中参与氨基酸代谢、糖酵解、三羧酸循环、丙酮酸盐代谢的蛋白最多。其中，氨基酰组氨酸二肽酶、嗜热菌状金属蛋白酶、尿刊酸水合酶和组氨酸氨裂解酶能有效降低烟叶中蛋白质。
위명학미생물제제MP제고연협품질적원인，이해제제적대사산물작위연구재료，채용액상색보천련질보법（LC-MS/MS）분석료미생물제제MP대사산물적단백질조성분。결과공감정출35개비중복단백질，단백등전점궤호균재4-7범위내，분자량균대우20 kD。KEGG대사통로분석현시，소감정적단백궤호도여대사유관，기중삼여안기산대사、당효해、삼최산순배、병동산염대사적단백최다。기중，안기선조안산이태매、기열균상금속단백매、뇨간산수합매화조안산안렬해매능유효강저연협중단백질。
Proteome of MP metabolic productions was analyzed by LC-MS/MS to determine the mechanism of MP in increasing tobacco quality. 35 non-repeated proteins were identified whose isoelectric point fall within the scope of 4 to 7 and molecular weight are more than 20 kD. KEGG analysis indicated that those proteins were involved in metabolic pathways especially in amino acid metabolism, glycolysis, tricarboxylic acid cycle and pyruvate metabolism. Enzymes such as aminoacyl-histidine dipeptidase, thermolysin-like metalloprotease, HutU urocanate hydratase and histidine ammonia-lyase, function in protein degradation were vital for increasing tobacco quality after MP spraying.