CAJ | 학술논문

在水温28℃条件下,以12 mg/kg剂量对罗非鱼单次口服给药，采用HPLC方法测定不同时间实验鱼肝脏和肾脏中的药物水平,分析氟苯尼考在罗非鱼肝、肾组织的吸收及消除规律。结果显示，肝脏和肾脏中的药动学参数均符合药动学一室模型，肝脏的药物消除速度快于肾脏,消除半衰期T1/2β分别为4.89 h和15.93 h；药物在肝脏的Tmax为5.43 h，吸收峰值为4.84μg/g；59 h后肝、肾组织中的药物含量均低于0.8μg/g。取12 mg/kg剂量连续给药7d的实验鱼肝、肾组织，进行组织学观察，结果显示，给药组罗非鱼的肝、肾组织均未出现病理性变化。此外，氟苯尼考对6株罗非鱼常见病原菌的体外抑菌实验中，最小抑菌浓度（MIC）均≤4 mg/L，表明常用剂量氟苯尼考在罗非鱼体内消除快、残留少且不造成组织损伤，对常见病原菌具有良好的抑菌效果。
재수온28℃조건하,이12 mg/kg제량대라비어단차구복급약，채용HPLC방법측정불동시간실험어간장화신장중적약물수평,분석불분니고재라비어간、신조직적흡수급소제규률。결과현시，간장화신장중적약동학삼수균부합약동학일실모형，간장적약물소제속도쾌우신장,소제반쇠기T1/2β분별위4.89 h화15.93 h；약물재간장적Tmax위5.43 h，흡수봉치위4.84μg/g；59 h후간、신조직중적약물함량균저우0.8μg/g。취12 mg/kg제량련속급약7d적실험어간、신조직，진행조직학관찰，결과현시，급약조라비어적간、신조직균미출현병이성변화。차외，불분니고대6주라비어상견병원균적체외억균실험중，최소억균농도（MIC）균≤4 mg/L，표명상용제량불분니고재라비어체내소제쾌、잔류소차불조성조직손상，대상견병원균구유량호적억균효과。
The pharmacokinetics and elimination of florfenicol in liver and kidney of tilapia (Oreochromis niloticus) single-orally administered 12 mg/kg of florfenicol at water temperature of 28 ℃were studied by HPLC in different periods. The results showed that the pharmacokinetic parameters in the liver and kidney were all in accord with the pharmacokinetic one-compartment model. The elimination speed of florfenicol in liver was faster than that in kidney,and the elimination half-life (T1/2β) of florfenicol in liver and kidney were 4.89 h and 15.93 h, respectively. The Tmax of florfenicol in liver was 5.43 h;59 hours after administration, the concentrations of florfenicol in liver and kidney were all less than 0.8 μg/g. Liver and kidney extracted from test fish continuous administrated with 12mg/kg florfenicol for 7 days were observed histologically. The results showed that no histopathological change was found in liver and kidney. The inhibitory effect of florfenicol to 6 kinds of pathogenic bacteria was detected, and the results showed MIC≤4 mg/L in all tested bacteria. This study indicated that florfenicol eliminate fast, has less residue and cause no histopathological damage in tilapia. As evidenced by the advantages above, it has good antibacterial effects on common pathogens in tilapia.