CAJ | 학술논문

分别用吖啶橙染色荧光显微计数法和平皿分离计数法对秋茄内生细菌总量和可培养内生细菌数量的时空变化进行测定。结果表明，秋茄植株根茎叶内均含有大量的内生细菌，其中根茎叶内生细菌总量平均分别为4.35×107 ind/g、6.31×107 ind/g和5.02×107 ind/g；可培养细菌总量平均分别为2.58×103 cfu/g、1.76×103 cfu/g和4.43×103 cfu/g。其时空变化动态为：内生细菌总量根部变化基本平稳，而叶部和茎部均呈峰形变化，其峰值分别出现在5月和7月；可培养细菌数量根茎叶均呈峰形变化，根部峰值出现在9月，而茎叶部峰值均出现在3月。秋茄植株可培养的内生细菌数量占其内生细菌总量比率为(0.02～8.33)×10-4，秋茄植株体内仅有小部分的内生细菌为可培养，绝大多数内生细菌为不可培养或难培养。
분별용아정등염색형광현미계수법화평명분리계수법대추가내생세균총량화가배양내생세균수량적시공변화진행측정。결과표명，추가식주근경협내균함유대량적내생세균，기중근경협내생세균총량평균분별위4.35×107 ind/g、6.31×107 ind/g화5.02×107 ind/g；가배양세균총량평균분별위2.58×103 cfu/g、1.76×103 cfu/g화4.43×103 cfu/g。기시공변화동태위：내생세균총량근부변화기본평은，이협부화경부균정봉형변화，기봉치분별출현재5월화7월；가배양세균수량근경협균정봉형변화，근부봉치출현재9월，이경협부봉치균출현재3월。추가식주가배양적내생세균수량점기내생세균총량비솔위(0.02～8.33)×10-4，추가식주체내부유소부분적내생세균위가배양，절대다수내생세균위불가배양혹난배양。
In this study, the total number of endogenous bacteria and the culturable endogenous bacteria in Kandelia candel collected from Zhanjiang Donghai island mangrove nature reserve were determined by acridine orange fluorescence microscopy and tablet separation, respectively. Statistical results of four seasons showed that K. candel in vivo owned numerous bacteria. The average total amount of bacteria in roots, stems and leaves were 4.35×107 ind/g, 6.31×107 ind/g and 5.02×107 ind/g successively, and the number of culturable corresponds to 2.58×103 cfu/g, 1.76×103 cfu/g and 4.43×103 cfu/g. Temporal dynamics regularity showed the total amount of endophytic bacteria in roots remained stable, and those in the leaves and stems reached maximum amount in July and May. As for the culturable bacteria, all of the donor materials were peak shape variation, maximum amount in roots appeared in September, and that in stems and leaves both emerged in March. Besides, culturable endophytic bacteria account for 0.002‰-0.833‰ of the total bacteria, It is indicated that the majority of culturable bacteria in the mangrove K candel in vivo were difficult to culture or uncultured. The results of this study would provide the basis for understanding temporal dynamics regularity of endogenous bacteria in mangrove and developing its resources .