CAJ | 학술논문

为了提高低温条件下沼气发酵的效率，本文利用Hungate厌氧操作技术从新疆库尔勒越冬沼气池污泥中分离出一株生长温度范围为8～40℃的兼性嗜冷产甲烷菌株ZG-2。该菌株革兰氏染色为阳性，呈不规则球状叠放在一起，不运动，能够利用H2/CO2混合物、甲醇、甲胺、乙酸盐作为单一碳源底物进行厌氧发酵生产甲烷。最适生长条件：温度为25～30℃，生长pH为6；NaCl浓度为0．2 mol/L；不能耐受链霉素和卡那霉素。根据菌株形态特征和生理生化特性以及16S rDNA序列分析，初步认为菌株ZG-2为兼性嗜冷巴氏产甲烷八叠球菌（Methanosarcina barkeri ）。
위료제고저온조건하소기발효적효솔，본문이용Hungate염양조작기술종신강고이륵월동소기지오니중분리출일주생장온도범위위8～40℃적겸성기랭산갑완균주ZG-2。해균주혁란씨염색위양성，정불규칙구상첩방재일기，불운동，능구이용H2/CO2혼합물、갑순、갑알、을산염작위단일탄원저물진행염양발효생산갑완。최괄생장조건：온도위25～30℃，생장pH위6；NaCl농도위0．2 mol/L；불능내수련매소화잡나매소。근거균주형태특정화생리생화특성이급16S rDNA서렬분석，초보인위균주ZG-2위겸성기랭파씨산갑완팔첩구균（Methanosarcina barkeri ）。
In order to improve the efficiency of biogas fermentation under low temperature conditions ,an amphipsychrophilic methanogen ZG-2 with growth temperature between 8 and 40℃ was isolated by Hungate tube technique from the sludge of methane tank in Korla ,Xinjiang ,China .The cells of strain ZG-2 were aggregated ,non-motile and gram-positive .This strain could use H2/CO2 mixture ,ethanol ,methylamine ,dimethylamine or trimethylamine as the sole carbon source for its grow th and methanogenesis .The optimum growth conditions were temperature of 25 to 30 ℃ ,pH 6 .0 and NaCl concentration of 0 .2 mol/L .It was sensitive to streptomycin and Kanamycin .Morphological and physio-biochemical characteristics combined with 16S rD-NA sequence analysis demonstrated that ZG-2 belonged to Methanosarcina barkeri .