CAJ | 학술논문

为探讨采用羧基化多壁碳纳米管（MWCNTs-COOH）非共价接枝神经生长因子（NGF）制备碳纳米管神经生长因子（MWCNTs-NGF）复合物，考察复合物的生物活性。采用透射电子显微镜（TEM）表征MWCNTs-NGF复合物的微观形貌，酶联免疫吸附法（ELISA法）测定MWCNTs-NGF复合物载带 NGF的量，MTT 法测定了MWC-NTs-NGF复合物的对嗜铬细胞瘤细胞（PC12细胞）的毒性，PC12细胞培养法评价复合物的生物活性，TEM 表征复合物与细胞的分布情况。结果：TEM图像表明NGF连接到了MWCNT上，ELISA法测得MWCNTs-NGF复合物载带NGF的量为797．63 pg/mg ，MWCNTs-NGF复合物对PC12细胞有一定的毒性，生物活性试验表明NGF浓度相同的情况下，MWCNTs-NGF复合物组PC12细胞的分化率明显高于 NGF组。TEM 图像表明碳纳米管能进入细胞。结论：碳纳米管能载带NGF进入细胞，使NGF能更好的表达生物活性。
위탐토채용최기화다벽탄납미관（MWCNTs-COOH）비공개접지신경생장인자（NGF）제비탄납미관신경생장인자（MWCNTs-NGF）복합물，고찰복합물적생물활성。채용투사전자현미경（TEM）표정MWCNTs-NGF복합물적미관형모，매련면역흡부법（ELISA법）측정MWCNTs-NGF복합물재대 NGF적량，MTT 법측정료MWC-NTs-NGF복합물적대기락세포류세포（PC12세포）적독성，PC12세포배양법평개복합물적생물활성，TEM 표정복합물여세포적분포정황。결과：TEM도상표명NGF련접도료MWCNT상，ELISA법측득MWCNTs-NGF복합물재대NGF적량위797．63 pg/mg ，MWCNTs-NGF복합물대PC12세포유일정적독성，생물활성시험표명NGF농도상동적정황하，MWCNTs-NGF복합물조PC12세포적분화솔명현고우 NGF조。TEM 도상표명탄납미관능진입세포。결론：탄납미관능재대NGF진입세포，사NGF능경호적표체생물활성。
Objective :To prepare complex of carbon nanotubes nerve growth factor (MWCNTs-NGF) through the method of non-covalently grafting ,and investigate the bioactivity of the complexes .Methods :We characterized the morphology of the MWCNTs-NGF complex using transmission electron microscopy(TEM) ,determined the amount of NGF that MWCNTs-NGF complex carried with the method of enzyme-linked immunosorbent assay(ELISA) ,evaluated the cytotoxicity of the complexes by the method of MTT ,evaluated the biological activity of the MWCNTs-NGF complex through cultured pheochromocytoma cells (PC12 cells) ,and characterized the distribution of the complex with the cells using TEM .Results :TEM images showed that NGF was connected to the MWCNTs ,the result of ELISA measured the amount of NGF that MWCNTs-NGF complex car-ried was 797 .63pg · mg ,MTT tests showed that the MWCNTs-NGF complex have some toxicity on PC12 cells ,biological ac-tivity tests showed that the differentiation of MWCNTs-NGF complex group on PC12 cells was significantly higher than the NGF group in the case of the same concentration of NGF ,and TEM images showed that carbon nanotubes could enter cells . Conclusion:Carbon nanotubes can carry NGF protein into the cells and help to promote nerve cell growth .