CAJ | 학술논문

为了探究棉花DELLA 蛋白基因GhGAI4a在拟南芥中的功能，构建植物表达载体 pBP35S∶GhGAI4a和pBP35S∶Ghgai4a ，利用农杆菌介导的花滴法将其转入Col野生型拟南芥。结果显示，2个载体各自获得6个独立的转基因拟南芥纯合株系。分别统计48-96 h种子萌发率，测量生长7 d后幼苗的主根长度，与非转基因植株相比，过量表达GhGAI4a、Ghgai4a对拟南芥种子萌发及主根生长具有明显的抑制作用。1μmol/L GA处理转基因植株， GhGAI4a转基因植株主根长和萌发率均增大，而Ghgai4a转基因植株主根长度和萌发率均无显著变化。
위료탐구면화DELLA 단백기인GhGAI4a재의남개중적공능，구건식물표체재체 pBP35S∶GhGAI4a화pBP35S∶Ghgai4a ，이용농간균개도적화적법장기전입Col야생형의남개。결과현시，2개재체각자획득6개독립적전기인의남개순합주계。분별통계48-96 h충자맹발솔，측량생장7 d후유묘적주근장도，여비전기인식주상비，과량표체GhGAI4a、Ghgai4a대의남개충자맹발급주근생장구유명현적억제작용。1μmol/L GA처리전기인식주， GhGAI4a전기인식주주근장화맹발솔균증대，이Ghgai4a전기인식주주근장도화맹발솔균무현저변화。
To analyze the function of cotton DELLA protein GhGAI4a gene in A rabidopsis thaliana ,we constructed the plant ex-pression vector pBP35S∶GhGAI4a and pBP35S∶Ghgai4a and converted them into A rabidopsis thaliana Col with the method of Agrobacterium-mediated inflorescence infection .Then through the kanamycin screening ,the test of polymerase chain reaction (PCR) technology and the semi-quantitative RT-PCR analysis ,we obtained six separate transgenic Arabidopsis homozygous from each of the two vectors .We made statistics about the 48-96 h seed germination rate ,and measured the taproot length of the 7 d seedling .Compared with non-transgenetic plants ,GhGAI4a and Ghgai4a have obvious inhibitory effect on seed germination and root growth .Meanwhile ,through 1 μmol/L GA treatment ,the taproot length and germination rate of GhGAI4a transgenic plants increased while the Ghgai4a transgenic plants did not change obviously .