CAJ | 학술논문

目的：使用口服他莫西芬法建立美国癌症研究所（ ICR）小鼠子宫腺肌病模型，并对此模型生育力情况进行评估。方法取24只新生的雌性ICR小鼠，于日龄第2天时开始口服他莫西芬，于日龄42天、90天时分别进行子宫组织形态学观察，于日龄90天时与正常性成熟雄鼠进行交配，妊娠第9天取出子宫组织，剖腹观察双侧子宫胚胎着床数。结果①子宫腺肌病模型小鼠在42日龄时出现子宫内膜间质浸润，肌层发育紊乱；②模型小鼠于90日龄时出现子宫腺肌病典型表现：子宫内膜侵入肌层，但尚未达浆膜下；③体内试验发现，与正常对照小鼠相比，模型小鼠生育力明显下降（Fisher’s值为0．000，P＜0．01）。结论口服他莫西芬法可成功诱导ICR小鼠子宫腺肌病模型，此模型小鼠生育力下降，可作为生殖医学领域子宫腺肌病研究较好的动物模型。
목적：사용구복타막서분법건립미국암증연구소（ ICR）소서자궁선기병모형，병대차모형생육력정황진행평고。방법취24지신생적자성ICR소서，우일령제2천시개시구복타막서분，우일령42천、90천시분별진행자궁조직형태학관찰，우일령90천시여정상성성숙웅서진행교배，임신제9천취출자궁조직，부복관찰쌍측자궁배태착상수。결과①자궁선기병모형소서재42일령시출현자궁내막간질침윤，기층발육문란；②모형소서우90일령시출현자궁선기병전형표현：자궁내막침입기층，단상미체장막하；③체내시험발현，여정상대조소서상비，모형소서생육력명현하강（Fisher’s치위0．000，P＜0．01）。결론구복타막서분법가성공유도ICR소서자궁선기병모형，차모형소서생육력하강，가작위생식의학영역자궁선기병연구교호적동물모형。
Objective To establish adenomyosis ( AM) model of mice by oral administration of tamoxifen to Institute of Cancer Research ( ICR) mice, and to study the fertility of the AM model .Methods Twenty-four newly born ICR mice with AM were induced by oral administration of tamoxifen from day 2 after birth.The histomorphology of uterus on day 42 and 90 was observed .The models of mice with AM were mated to normal male mice on day 90, and the implantation of embryos in uterus on 9th pregnant day was observed .Results There was infiltration of stroma in the endometrium and disordered development in tunica muscularis in the uterus of model mice on day 42. There were typical manifestations of AM in model mice on day 90, endometrium infiltrating muscular layer but not reaching the subserosa . In vivo experiment, a significantly declining of fertility in AM model mice was found when they were compared with control mice (Fisher’ s value was 0.000, P<0.01).Conclusion Oral administration of tamoxifen can induce the mouse model of AM successfully .The fertility of AM mice declines significantly , and they can be taken as ideal animal models to study the disease of AM in reproductive area .