CAJ | 학술논문

为了利用杂交瘤技术制备抗3α-HSD单克隆抗体，用表达纯化的3α-HSD作为抗原免疫Balb/c小鼠，获得针对该抗原致敏的B淋巴细胞，并用获得的B淋巴细胞与SP2/0进行细胞融合，得到杂交瘤细胞株，在ELISA间接法筛选出阳性孔的基础上，进行亚克隆，筛选出能稳定分泌抗3α-HSD单克隆抗体的杂交瘤细胞株。结果得到能分泌抗3α-HSD单克隆抗体的杂交瘤细胞株。制备过程中发现很多特殊的现象，如杂交瘤细胞上清与单抗腹水效价过低，但多抗血清效价较高等。文章对在单抗的制备过程中遇到的问题做了初步分析，为之后制备抗3α-HSD单克隆抗体的研究提供技术支持。
위료이용잡교류기술제비항3α-HSD단극륭항체，용표체순화적3α-HSD작위항원면역Balb/c소서，획득침대해항원치민적B림파세포，병용획득적B림파세포여SP2/0진행세포융합，득도잡교류세포주，재ELISA간접법사선출양성공적기출상，진행아극륭，사선출능은정분비항3α-HSD단극륭항체적잡교류세포주。결과득도능분비항3α-HSD단극륭항체적잡교류세포주。제비과정중발현흔다특수적현상，여잡교류세포상청여단항복수효개과저，단다항혈청효개교고등。문장대재단항적제비과정중우도적문제주료초보분석，위지후제비항3α-HSD단극륭항체적연구제공기술지지。
Hybridoma technology was used to prepare 3α-HSD monoclonal antibody. Using 3α-HSD to express and pu-rify as antigen to immune Balb/c mice, the sensitization of B lymphocytes that against the antigen was gained. The hybridoma cell lines is got by fusing the B lymphocyte cell with SP2/0. Screen positive hole in ELISA, then the cell lines is cloned and the last is to screen the cell lines stabling to secrete 3α-HSD monoclonal antibody hybridoma. Al-though cell lines which could secrete monoclonal antibody hybridoma against 3α-HSD have been gotten, many special phenomenons were discovered during the preparation, such as qing on hybridoma with single ascites titer resistance is too low, but high serum titer. In the process of preparing single resistance, a preliminary analysis for against 3α-HSD monoclonal antibody preparation were made and technical support would be provided.