中外医学研究
中外醫學研究
중외의학연구
CHINESE AND FOREIGN MEDICAL RESEARCH
2013年
36期
1-2,3
,共3页
脂肪干细胞%口腔上皮细胞%组织工程%共培养
脂肪榦細胞%口腔上皮細胞%組織工程%共培養
지방간세포%구강상피세포%조직공정%공배양
Adipose tissue derired stem%Oral keratinocyte%The tissue engineering%Co-culture environment
目的：探讨体外共培养环境对犬脂肪干细胞(ADSC)、口腔上皮细胞(OK)移行速率、增殖速率的影响。方法：获取犬ADSC和OK并鉴定，将两种细胞种植于同一个刻度培养皿内，检测共培养环境下细胞的移行速率，与单一细胞培养环境作对比，观察细胞移行速率的改变。收集两种细胞培养上清，加入到对方培养基中，形成体外模拟混合培养环境，MTT法检测细胞增殖曲线的改变。结果：共培养环境下，OK、ADSC细胞的移行速率均较单一细胞培养环境下高。与常规培养相比，在体外模拟共培养环境下，OK、ADSC细胞的增殖曲线均变陡。结论：在体外共培养环境中， ADSC、OK呈现互相促进、互相吸引、协同增殖态势，细胞的移行速率、增殖速率均得到提高，能够共同用于组织工程口腔黏膜的构建。
目的：探討體外共培養環境對犬脂肪榦細胞(ADSC)、口腔上皮細胞(OK)移行速率、增殖速率的影響。方法：穫取犬ADSC和OK併鑒定，將兩種細胞種植于同一箇刻度培養皿內，檢測共培養環境下細胞的移行速率，與單一細胞培養環境作對比，觀察細胞移行速率的改變。收集兩種細胞培養上清，加入到對方培養基中，形成體外模擬混閤培養環境，MTT法檢測細胞增殖麯線的改變。結果：共培養環境下，OK、ADSC細胞的移行速率均較單一細胞培養環境下高。與常規培養相比，在體外模擬共培養環境下，OK、ADSC細胞的增殖麯線均變陡。結論：在體外共培養環境中， ADSC、OK呈現互相促進、互相吸引、協同增殖態勢，細胞的移行速率、增殖速率均得到提高，能夠共同用于組織工程口腔黏膜的構建。
목적：탐토체외공배양배경대견지방간세포(ADSC)、구강상피세포(OK)이행속솔、증식속솔적영향。방법：획취견ADSC화OK병감정，장량충세포충식우동일개각도배양명내，검측공배양배경하세포적이행속솔，여단일세포배양배경작대비，관찰세포이행속솔적개변。수집량충세포배양상청，가입도대방배양기중，형성체외모의혼합배양배경，MTT법검측세포증식곡선적개변。결과：공배양배경하，OK、ADSC세포적이행속솔균교단일세포배양배경하고。여상규배양상비，재체외모의공배양배경하，OK、ADSC세포적증식곡선균변두。결론：재체외공배양배경중， ADSC、OK정현호상촉진、호상흡인、협동증식태세，세포적이행속솔、증식속솔균득도제고，능구공동용우조직공정구강점막적구건。
Objective:To investigate the influence of vitro co-culture environment on the migration and proliferation patterns of canine adipose-tissue derived stem cell and oral keratinocytes.Method:Obtain canine ADSC,OK and make the identification.Seed the two cell lines onto a scaled dish,record the migration rate of the two cell lines in co-culture environment,and compare it with the record in monoculture environment.Collect the supernatant of the two cell lines,and add it to the other’s culture medium to simulate a co-culture environment.Detect the changes in cell proliferation curves through MTT assay.Result:The migration rate of the ADSC and OK improved in co-culture environment.The proliferation curves of the two cell line were steeper in co-culture environment than in monoculture environment.Conclusion:In vitro co-culture environment,ADSC,OK exhibit mutual promotion,mutual attraction,collaborative proliferation situation,cell migration rate and proliferation rate are improved.They can be used together for the construction of the tissue engineering oral mucosa.
