CAJ | 학술논문

苯并(a)芘（Benzo(a)pyrene，BaP）是一种含有5个苯环的多环芳烃，是环境中广泛存在的一类有机污染物，苯并(a)芘可引起多种形式的DNA损伤。彗星试验，又称为单细胞凝胶电泳(Single Cell Gel Electrophoresis，SCGE)，是一项灵敏、快速的在单细胞水平检测DNA链断裂的技术，已经广泛应用于水环境的生物监测中。污染物的毒性监测与评估一直是环境科学研究的热点问题之一。本文通过生态毒理学试验，利用单细胞凝胶电泳技术，研究不同浓度苯并(a)芘暴露对罗非鱼（GIFT Oreochromis niloticus）肝细胞DNA的损伤情况。试验将罗非鱼在0.1、1、10、50μg·L-14个苯并(a)芘(BaP)浓度下分别暴露4、7、14 d，利用彗星试验研究BaP对罗非鱼肝细胞DNA的损伤情况，以尾部DNA含量、尾长、尾矩及Olive尾矩为评价指标，结果表明，BaP会对肝细胞DNA造成不同程度的损伤：在0.1、1、10μg·L-13个浓度组，DNA损伤随着BaP暴露浓度的增加而增加，呈现一定的剂量效应关系，50μg·L-1组DNA损伤有所下降。在时间上，除1μg·L-1剂量组的尾部DNA含量、10μg·L-1组的尾部DNA含量及Olive尾矩外，其他指标有在第7天降低之后又升高的趋势，这意味着肝细胞DNA损伤可在一定程度上反映水体中BaP的污染情况。该实验为进一步探讨苯并(a)芘的致癌机制及环境中苯并(a)芘的监测提供一定的科学依据。
분병(a)비（Benzo(a)pyrene，BaP）시일충함유5개분배적다배방경，시배경중엄범존재적일류유궤오염물，분병(a)비가인기다충형식적DNA손상。혜성시험，우칭위단세포응효전영(Single Cell Gel Electrophoresis，SCGE)，시일항령민、쾌속적재단세포수평검측DNA련단렬적기술，이경엄범응용우수배경적생물감측중。오염물적독성감측여평고일직시배경과학연구적열점문제지일。본문통과생태독이학시험，이용단세포응효전영기술，연구불동농도분병(a)비폭로대라비어（GIFT Oreochromis niloticus）간세포DNA적손상정황。시험장라비어재0.1、1、10、50μg·L-14개분병(a)비(BaP)농도하분별폭로4、7、14 d，이용혜성시험연구BaP대라비어간세포DNA적손상정황，이미부DNA함량、미장、미구급Olive미구위평개지표，결과표명，BaP회대간세포DNA조성불동정도적손상：재0.1、1、10μg·L-13개농도조，DNA손상수착BaP폭로농도적증가이증가，정현일정적제량효응관계，50μg·L-1조DNA손상유소하강。재시간상，제1μg·L-1제량조적미부DNA함량、10μg·L-1조적미부DNA함량급Olive미구외，기타지표유재제7천강저지후우승고적추세，저의미착간세포DNA손상가재일정정도상반영수체중BaP적오염정황。해실험위진일보탐토분병(a)비적치암궤제급배경중분병(a)비적감측제공일정적과학의거。
Benzo(a)pyrene (BaP), with five benzo-rings,is an important isomers of polycyclic aromatic hydrocarbons (PAHs), and it is a prevalent pollution substances in environment. The mechanism of environmental toxicology of BaP is DNA damage. Comet assay, named Single Cell Gel Electrophoresis (SCGE) is a sensitive, fast technology to detect DNA ring damage;this technology is broadly applied to bio-monitor in aquatic environment. The toxicological monitoring and assessment of pollutant is always a hotspot of environmental research. This eco-toxicological experiment focused on the DNA damage of tilapia (GIFT Oreochromis niloticus) when exposed to BaP via comet assay.Tilapia (GIFT Oreochromis niloticus) were exposed to different concentrations of ben-zo(a)pyrene (0.1 μg·L-1 ,1 μg·L-1 ,0 μg·L-1 , 50 μg·L-1 ) for 4 d, 7 d and 14 d respectively. DNA damage in liver cells of tilapia was evaluated by comet assay. Tail DNA content, tail length and tail moment, Olive tail moment were used to evaluate DNA damage. Results showed that BaP exposure could cause liver cell DNA damage in tilapia. Among the concentration of 0.1 μg·L-1, 1 μg·L-1, 10 μg·L-1, DNA damage increased following the BaP exposed concentrations, but decreased in 50μg·L-1 group. In addition to the tail DNA content of 1 μg·L-1 dose group, tail DNA and Olive tail moment of 10μg·L-1 group, other parameters decreased in 7 d and then increased. These results demonstrated that liver cell DNA damage can reflect BaP pollution of water environment to a certain extent. The experiment provided a detailed data to understand the mechnism and environmental monitoring of BaP.