CAJ | 학술논문

对斜纹夜蛾核多角体病毒（Spodoptera litura nucleopolyhedrovirus， Spli NPV）郴州株的室内生物活性进行了分析并对其田间应用效果进行了系统研究。该病毒的人工繁殖方法以饲喂法较好，病死率为65.0%；害虫染毒后4～8d达死亡高峰，高温、高湿、寡日照天气有利于病毒的发生流行；此病毒的室内校正防效为86.6%，略优于已投入市场的病毒产品；田间校正防效为88.4%，稍高于阿维菌素农药产品的防效；病毒原液中的病毒多角体的浓度为1.03×101 OBs/ml，为国内的强毒株。该病毒作为防治害虫的生物因子，具有很好的应用开发前景。
대사문야아핵다각체병독（Spodoptera litura nucleopolyhedrovirus， Spli NPV）침주주적실내생물활성진행료분석병대기전간응용효과진행료계통연구。해병독적인공번식방법이사위법교호，병사솔위65.0%；해충염독후4～8d체사망고봉，고온、고습、과일조천기유리우병독적발생류행；차병독적실내교정방효위86.6%，략우우이투입시장적병독산품；전간교정방효위88.4%，초고우아유균소농약산품적방효；병독원액중적병독다각체적농도위1.03×101 OBs/ml，위국내적강독주。해병독작위방치해충적생물인자，구유흔호적응용개발전경。
The laboratory bioassay and field control efficacy of Spodoptera litura nu-cleopolyhedrovirus (Spli NPV) Chenzhou strain were preliminarily examined. The effi-cient artificial propagation method was to feed the host larvae with virus suspension, and the average mortality of the insects was 65.0%. The death peak of the pests appeared 4-8 d after virus infection. The high temperature, high humidity and poor light could help the virus infection and propagation. Filed control efficacy of Chen-zhou strain was 86.6% in laboratory, which was better than of another commercial strain. The corrected control efficacy of this strain was 88.4% the field, which was higher than that of avermectin pesticide significantly. It was detected that the occlu-sion body (OB) concentration of the initial virus’s stock solution was 1.03×1011 OBs/ml, and it was a strong SpliNPV strain, as it showed an excellent efficacy to control the pest Spodoptera litura, and thus there wil be a good prospect of application and development of this SpliNPV strain.