CAJ | 학술논문

[目的]通过腹腔注射的致敏方式建立中国龙虾食物变态反应 Balb/c小鼠模型，探讨中国龙虾食物变态反应体外鉴定与评价方法。[方法]将40只雄性 Balb/c小鼠分为卵清蛋白(OVA)阳性对照组、Coca’ s液阴性对照组、空白对照组和模型组，以 OVA、中国龙虾粗提蛋白，加氢氧化铝佐剂腹腔注射免疫Balb/c小鼠，建立食物变态反应小鼠模型。ELISA法测定第二次致敏激发后血清中 IgE与组胺水平并进行被动皮肤过敏试验（ PCA）确定特异性IgE抗体滴度，同时观察脾指数、小肠组织学变化及激发后的食物变态反应症状。[结果]末次激发后1 h采血，中国龙虾粗提蛋白组血清IgE含量为236.75(±73.39)μg/L，与阳性对照 OVA 组无区别，与阴性对照Coca’s液组和正常对照组相比显著升高（P<0.01）；中国龙虾粗提蛋白组的组胺含量406.55(±232.79）μg/L与正常对照组相比显著升高(P<0.01)；PCA反应中国龙虾粗提蛋白组血清特异性 IgE抗体滴度达到1/16；中国龙虾粗提蛋白组和 OVA致敏组小鼠脾指数明显大于 Coca’s液或正常对照组(P<0.01)且前两组小肠粘膜固有层皆出现淋巴细胞、嗜酸性粒细胞等炎性细胞浸润。[结论]建立了一种中国龙虾食物变态反应小鼠模型，通过 ELISA测定血清 IgE和组胺以及 PCA确定特异性 IgE抗体滴度可作为一种鉴定与评价中国龙虾食物变态反应的方法。
[목적]통과복강주사적치민방식건립중국룡하식물변태반응 Balb/c소서모형，탐토중국룡하식물변태반응체외감정여평개방법。[방법]장40지웅성 Balb/c소서분위란청단백(OVA)양성대조조、Coca’ s액음성대조조、공백대조조화모형조，이 OVA、중국룡하조제단백，가경양화려좌제복강주사면역Balb/c소서，건립식물변태반응소서모형。ELISA법측정제이차치민격발후혈청중 IgE여조알수평병진행피동피부과민시험（ PCA）학정특이성IgE항체적도，동시관찰비지수、소장조직학변화급격발후적식물변태반응증상。[결과]말차격발후1 h채혈，중국룡하조제단백조혈청IgE함량위236.75(±73.39)μg/L，여양성대조 OVA 조무구별，여음성대조Coca’s액조화정상대조조상비현저승고（P<0.01）；중국룡하조제단백조적조알함량406.55(±232.79）μg/L여정상대조조상비현저승고(P<0.01)；PCA반응중국룡하조제단백조혈청특이성 IgE항체적도체도1/16；중국룡하조제단백조화 OVA치민조소서비지수명현대우 Coca’s액혹정상대조조(P<0.01)차전량조소장점막고유층개출현림파세포、기산성립세포등염성세포침윤。[결론]건립료일충중국룡하식물변태반응소서모형，통과 ELISA측정혈청 IgE화조알이급 PCA학정특이성 IgE항체적도가작위일충감정여평개중국룡하식물변태반응적방법。
[Objective] This study aimed to establish Balb/c mice model for food al er-gy caused by Chinese lobsters through using intraperitoneal injection for sensitization and explore methods for in vitro identification and evaluation of food al ergy caused by Chinese lobsters. [Method] The 40 male Bal/c mice were divided into ovalbumin (OVA) positive control group, Coca’s solution negative control group, blank control group and model group. Balb/c mice model was established by intraperitoneal y in-jection of immunized Balb/c mice with OVA or Chinese lobster crude protein with alu-minum hydroxide adjuvant. IgE and histamine levels in serum after the second chal enge were determined by ELISA method, and the specific IgE antibody titer was determined by passive cutaneous anaphylaxis test (PCA); additional y, spleen index and histological changes in the smal intestine, as wel as food al ergy symp-toms after chal enge were also calculated or observed. [Results] After the last chal-lenge, IgE content was (236.75 ±73.39) μg/L in the Chinese lobster crude protein group, revealing no difference with that in the OVA group, but significantly higher than that in either the Coca’s solution group or the blank control group (P<0.01);histamine content in serum in the Chinese lobster crude protein group was (406.55± 232.79), significantly higher than that in the blank control group(P<0.01). In the pas-sive cutaneous anaphylaxis test, IgE antibody titer reached 1/16 after the last chal-lenge in the Chinese lobster crude protein group. Spleen index in both Chinese lob-ster crude protein group and OVA group was significantly greater than that in either the Coca's solution group or the blank control group (P<0.01). What’s more, infil-tration of inflammatory cells like lymphocytes and eosnophils at the lamina propria of intestinal mucosa was also observed both Chinese lobster crude protein group and OVA group. [Conclusion] This study established Balb/c mice model for food al ergy caused by Chinese lobsters; serum IgE and ELISA assay and specific IgE antibody titer in PCA test can be used for the in vitro identification and evaluation of food al-lergies caused by Chinese lobsters.