浙江中医药大学学报
浙江中醫藥大學學報
절강중의약대학학보
JOURNAL OF ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2013年
10期
1230-1235
,共6页
糖尿病肾病%蛋白尿%肾功能%P钙黏蛋白%成纤维细胞特异蛋白1
糖尿病腎病%蛋白尿%腎功能%P鈣黏蛋白%成纖維細胞特異蛋白1
당뇨병신병%단백뇨%신공능%P개점단백%성섬유세포특이단백1
diabetic nephropathy%proteinuria%renal function%P-cadherin%fibroblast-specific protein 1
[目的]通过观察清热益气通络方对糖尿病肾病大鼠肾小球P钙黏蛋白(P-cadherin)、成纤维细胞特异蛋白1(fibroblast-fpecific protein 1,FSP1)表达影响，探索该方治疗DN蛋白尿的机制。[方法]将SD大鼠随机分为正常组和糖尿病肾病造模组(采用单侧肾切除腹腔注射链脲佐菌素的方法建立大鼠糖尿病肾病模型。将造模成功的大鼠再分为模型对照组、中药组和厄贝沙坦组。干预治疗12w后处死大鼠，分别检测各组大鼠24h尿蛋白量（urine protein, UPro）、血肌酐（serum creatinine ,Scr）、内生肌酐清除率（creatinine clearance rate, Ccr）、尿素氮（blood urea nitrogen ,BUN），血糖、血脂，免疫组织化学染色的方法观察肾小球P-cadherin、FSP1蛋白的表达。光镜及电镜观察肾小球结构。[结果]①与模型组比，中药组大鼠24h UPro、Ccr、BUN显著降低（P<0.01,P<0.05）。②与模型组比，中药组大鼠肾小球P-cadherin表达显著增高（P<0.01），FSP1阳性表达明显降低（P<0.01）；与厄贝沙坦组比，中药组FSP1阳性表达明显降低（P<0.05）。[结论]通过上调P-cadherin蛋白表达及降低FSP1蛋白的表达，部分拮抗足细胞上皮间充质转分化可能是清热益气通络中药减轻糖尿病肾病蛋白尿排泄重要作用点之一。
[目的]通過觀察清熱益氣通絡方對糖尿病腎病大鼠腎小毬P鈣黏蛋白(P-cadherin)、成纖維細胞特異蛋白1(fibroblast-fpecific protein 1,FSP1)錶達影響，探索該方治療DN蛋白尿的機製。[方法]將SD大鼠隨機分為正常組和糖尿病腎病造模組(採用單側腎切除腹腔註射鏈脲佐菌素的方法建立大鼠糖尿病腎病模型。將造模成功的大鼠再分為模型對照組、中藥組和阨貝沙坦組。榦預治療12w後處死大鼠，分彆檢測各組大鼠24h尿蛋白量（urine protein, UPro）、血肌酐（serum creatinine ,Scr）、內生肌酐清除率（creatinine clearance rate, Ccr）、尿素氮（blood urea nitrogen ,BUN），血糖、血脂，免疫組織化學染色的方法觀察腎小毬P-cadherin、FSP1蛋白的錶達。光鏡及電鏡觀察腎小毬結構。[結果]①與模型組比，中藥組大鼠24h UPro、Ccr、BUN顯著降低（P<0.01,P<0.05）。②與模型組比，中藥組大鼠腎小毬P-cadherin錶達顯著增高（P<0.01），FSP1暘性錶達明顯降低（P<0.01）；與阨貝沙坦組比，中藥組FSP1暘性錶達明顯降低（P<0.05）。[結論]通過上調P-cadherin蛋白錶達及降低FSP1蛋白的錶達，部分拮抗足細胞上皮間充質轉分化可能是清熱益氣通絡中藥減輕糖尿病腎病蛋白尿排洩重要作用點之一。
[목적]통과관찰청열익기통락방대당뇨병신병대서신소구P개점단백(P-cadherin)、성섬유세포특이단백1(fibroblast-fpecific protein 1,FSP1)표체영향，탐색해방치료DN단백뇨적궤제。[방법]장SD대서수궤분위정상조화당뇨병신병조모조(채용단측신절제복강주사련뇨좌균소적방법건립대서당뇨병신병모형。장조모성공적대서재분위모형대조조、중약조화액패사탄조。간예치료12w후처사대서，분별검측각조대서24h뇨단백량（urine protein, UPro）、혈기항（serum creatinine ,Scr）、내생기항청제솔（creatinine clearance rate, Ccr）、뇨소담（blood urea nitrogen ,BUN），혈당、혈지，면역조직화학염색적방법관찰신소구P-cadherin、FSP1단백적표체。광경급전경관찰신소구결구。[결과]①여모형조비，중약조대서24h UPro、Ccr、BUN현저강저（P<0.01,P<0.05）。②여모형조비，중약조대서신소구P-cadherin표체현저증고（P<0.01），FSP1양성표체명현강저（P<0.01）；여액패사탄조비，중약조FSP1양성표체명현강저（P<0.05）。[결론]통과상조P-cadherin단백표체급강저FSP1단백적표체，부분길항족세포상피간충질전분화가능시청열익기통락중약감경당뇨병신병단백뇨배설중요작용점지일。
[Objective]To study the effect of Qingre Yiqi Tongluo(QRYQTL) preseription on proteinria in diabetic nephropathy(DN) rats by means of ob-servation on the expressions of fibroblast-specific protein 1(FSP1) and P-cadherin in in glomerulus in rats.[Methods]60 Sprague-Dawleg rats were divided into normal control group(N), Diabetic nephropathy model group(M), QRYQTL preseription treatment group(Q) and Irbesartan treatment group(I). DN model rats were prepared by intraperitoneal injection with STZ after unilateral nephrectomy. The rats were sacrificed after 12 weeks of the treatment. The creatinine clearance(Ccr),blood urea nitrogen(BUN) and 24h urinary protein(UPro) were tested. Immunohistochemical staining was performed to explore the expressions of P-cadherin and fsp1 in glomerulus.[Results] ①Compared with M group, the levels of 24-hour upro in Q group were significantly de-creased(P<0.01).The levels of Ccr and BUN in Q group were both lower than those of M group( P<0.05). ② Compared with M group, the expressions of P-cadherin in glomerulus in Q group were increased( P<0.01) and the expressions of FSP1 were obviously decreased( P<0.01). The expressions of FSP1 in Q group were decreased compared with that of I groups(P<0.05).[Conclusions]QRYQTL preseription weakened the epithelial-mesenchymal transition (EMT) of podocyte by means of increasing the expression of P-cadherin and reducing the expression of FSP1, thereby decreased the Upro and protect the renal function.
