CAJ | 학술논문

[目的]采用 SRAP分子标记技术对5个新疆红花品种进行分析，探讨品种间的亲缘关系，为红花种质资源的保护和发掘利用提供科学依据。[方法]利用 SRAP分子标记方法对新疆5个红花品种和1个云南红花品种的基因组 DNA 进行分析。[结果]筛选出12对SRAP引物组合，对6份材料共扩增出171条清晰可用条带，其中多态性条带93条，多态性比率54.4%。6份材料的遗传相似系数变化范围在0.60~0.92。[结论]SRAP分子标记技术适合用于红花品种的亲缘关系研究和指导分子育种。
[목적]채용 SRAP분자표기기술대5개신강홍화품충진행분석，탐토품충간적친연관계，위홍화충질자원적보호화발굴이용제공과학의거。[방법]이용 SRAP분자표기방법대신강5개홍화품충화1개운남홍화품충적기인조 DNA 진행분석。[결과]사선출12대SRAP인물조합，대6빈재료공확증출171조청석가용조대，기중다태성조대93조，다태성비솔54.4%。6빈재료적유전상사계수변화범위재0.60~0.92。[결론]SRAP분자표기기술괄합용우홍화품충적친연관계연구화지도분자육충。
[Objective] This study aimed to analyze the genetic relationship of the Carthamus tinctorius L (safflower) varieties in Xinjiang, and thus to provide a scien-tific basis for its protection and development. [Method] The genomic DNA of five safflower varieties from Xinjiang and one from Yunnan were analyzed and compared by SRAP molecular marker. [Result] Twelve pairs of SRAP primers were selected to amplify the genomic DNA of the six materials. Among the 171 clear DNA bands final y obtained, 93 were polymorphic, accounting for 54.4% of total. Genetic similari-ty coefficient of the six safflower varieties ranged from 0.60 to 0.92. [Conclusion] SRAP molecular marker is suitable for safflower varieties research and to guide the molecular breeding.