CAJ | 학술논문

[目的]建立茄子愈伤组织悬浮细胞培养体系。[方法]以三月茄为试验材料，利用正交设计筛选适合茄子愈伤组织诱导、继代保存以及悬浮培养的培养基并测定悬浮培养过程中细胞生长状况。[结果]适宜茄子愈伤组织诱导的培养基为 MS+6-BA (0.2 mg/L)+NAA （0.2 mg/L）；适宜继代保存的培养基为 MS+6-BA（0.2 mg/L）+NAA（0.2 mg/L）+KT（0.1 mg/L）；适宜悬浮细胞培养的培养基为 MS+NAA（0.4 mg/L）+6-BA（0.2 mg/L）液体培养基。茄子悬浮培养细胞生长呈 S形曲线，其中前3d为起始期，3~7 d处于对数生长期，7~8 d为静止期；8~11 d为衰退期。悬浮细胞密度随着培养时间的延长增长，在培养第7天达到最高值，约3.8×105个/ml.随后细胞数量迅速减少。起始期细胞活力最高。茄子悬浮细胞在 MS+NAA （0.4 mg/L）+6-BA（0.2 mg/L）液体培养基上生长良好，其分裂指数最大达4.1%。[结论]茄子悬浮细胞培养体系为茄子生物技术研究提供了一条途径，可应用该体系进行遗传转化等方面的研究。
[목적]건립가자유상조직현부세포배양체계。[방법]이삼월가위시험재료，이용정교설계사선괄합가자유상조직유도、계대보존이급현부배양적배양기병측정현부배양과정중세포생장상황。[결과]괄의가자유상조직유도적배양기위 MS+6-BA (0.2 mg/L)+NAA （0.2 mg/L）；괄의계대보존적배양기위 MS+6-BA（0.2 mg/L）+NAA（0.2 mg/L）+KT（0.1 mg/L）；괄의현부세포배양적배양기위 MS+NAA（0.4 mg/L）+6-BA（0.2 mg/L）액체배양기。가자현부배양세포생장정 S형곡선，기중전3d위기시기，3~7 d처우대수생장기，7~8 d위정지기；8~11 d위쇠퇴기。현부세포밀도수착배양시간적연장증장，재배양제7천체도최고치，약3.8×105개/ml.수후세포수량신속감소。기시기세포활력최고。가자현부세포재 MS+NAA （0.4 mg/L）+6-BA（0.2 mg/L）액체배양기상생장량호，기분렬지수최대체4.1%。[결론]가자현부세포배양체계위가자생물기술연구제공료일조도경，가응용해체계진행유전전화등방면적연구。
[Objective] The aim of this article is to establish a cellsuspension culture system for eggplant. [Method] Using orthogonal design, appropriate media were screened for cal us induction, subculture and cellsuspension culture. [Result] The appropriate medium for cal us induction was MS supplemented with 0.2 mg/L 6-BA and 0.2 mg/L NAA; the appropriate subculture medium was MS supplemented with 0.2 mg/L 6-BA, 0.2 mg/L NAA, and 0.1 mg/L KT; the suitable medium for cellsus-pension culture was liquid MS medium supplemented with 0.4 mg/L NAA and 0.2 mg/L. cellgrowth curve was similar to "S" shape in the above suspension medium. The cellgrowth included four phases, the initial phase (1-3 d), the logarithmic phase (3-7 d), the steady phase (7-8 d), and the decline phase (8-11 d). With the increasing culture time, the number of suspension cells increased, and it reached the maximum value at the 7th d, about 3.8 ×105 cells/ml. Then the number of cells began to decline rapidly. The cellvigor was the highest at the initial phase. Sus-pension cells grew best in the liquid MS medium supplemented with NAA (0.4 mg/L) and 6-BA (0.2 mg/L). The mitotic index reached the maximum, about 4.1% in the above medium, which suggested that this medium was suitable for cellsuspension culture of eggplant. [Conclusion] cellsuspension culture system of eggplant provides a significant method for eggplant biotechnology. Genetic transformation and mutants screening can be carried out with this system.