CAJ | 학술논문

采用 ISSR分子标记技术对48个引种和选育的油橄榄品种进行遗传多样性和聚类分析。11条筛选出的引物共扩增出106条DNA谱带，其中有99条为多态性条带，多态位点百分率为93.40%。数据表明所分析的油橄榄种质具有丰富的遗传多样性。根据各品种的 Nei’s 遗传距离，运用 UPGMA方法构建了所有品种的聚类图，结果48个油橄榄品种被聚成了4个大类；84.6%的国内选育品种聚于2个类群中；多数引种品种并没有按照地理起源而是依据引种材料来源地和主要用途聚类。研究结果为油橄榄种质资源的利用以及进一步的良种选育提供参考。
채용 ISSR분자표기기술대48개인충화선육적유감람품충진행유전다양성화취류분석。11조사선출적인물공확증출106조DNA보대，기중유99조위다태성조대，다태위점백분솔위93.40%。수거표명소분석적유감람충질구유봉부적유전다양성。근거각품충적 Nei’s 유전거리，운용 UPGMA방법구건료소유품충적취류도，결과48개유감람품충피취성료4개대류；84.6%적국내선육품충취우2개류군중；다수인충품충병몰유안조지리기원이시의거인충재료래원지화주요용도취류。연구결과위유감람충질자원적이용이급진일보적량충선육제공삼고。
Inter-simple sequence repeat (ISSR) molecular markers were applied to an-alyze the genetic diversity and clustering of 48 introduced and bred cultivars of Olea euyopaea L. Total y 106 DNA bands were amplified by 11 screened primers, includ-ing 99 polymorphic bands; the percentage of polymorphic loci was 93.40%, indicat-ing a rich genetic diversity in Olea euyopaea L. germplasm resources. Based on Nei’s genetic distances between various cultivars, a dendrogram of 48 cultivars of Olea euyopaea L. was constructed using unweighted pair-group (UPMGA)method, which showed that 48 cultivars were clustered into four main categories; 84.6% of native cultivars were clustered into two categories; most of introduced cultivars were clustered based on their sources and main usages but not on their geographic ori-gins. This study wil provide references for the utilization and further genetic im-provement of Olea euyopaea L. germplasm resources.