农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2013年
9期
1212-1214
,共3页
DNA条形码%苍耳属%ITS%ITS2%rbcL
DNA條形碼%蒼耳屬%ITS%ITS2%rbcL
DNA조형마%창이속%ITS%ITS2%rbcL
DNA barcoding%Xanthium%ITS%ITS2%rbcL
[目的]为植物 DNA条形码标准基因筛选研究提供参考，减少植物 DNA条形码研究中的工作量。[方法]对7种苍耳属植物 ITS、ITS2及 rbcL基因采用相同的扩增条件（95℃4 min；[35 cycles：94℃30 s；52℃45 s；72℃45 s]；72℃10 min；4℃保存）。[结果]3种DNA条形码基因同时成功扩增。[结论]这说明植物 DNA条形码基因中 ITS、ITS2及 rbcL的PCR条件存在合并可能性。
[目的]為植物 DNA條形碼標準基因篩選研究提供參攷，減少植物 DNA條形碼研究中的工作量。[方法]對7種蒼耳屬植物 ITS、ITS2及 rbcL基因採用相同的擴增條件（95℃4 min；[35 cycles：94℃30 s；52℃45 s；72℃45 s]；72℃10 min；4℃保存）。[結果]3種DNA條形碼基因同時成功擴增。[結論]這說明植物 DNA條形碼基因中 ITS、ITS2及 rbcL的PCR條件存在閤併可能性。
[목적]위식물 DNA조형마표준기인사선연구제공삼고，감소식물 DNA조형마연구중적공작량。[방법]대7충창이속식물 ITS、ITS2급 rbcL기인채용상동적확증조건（95℃4 min；[35 cycles：94℃30 s；52℃45 s；72℃45 s]；72℃10 min；4℃보존）。[결과]3충DNA조형마기인동시성공확증。[결론]저설명식물 DNA조형마기인중 ITS、ITS2급 rbcL적PCR조건존재합병가능성。
[Objective] This study aimed to provide reference and reduce the workload for screening standard DNA barcoding genes of plants. [Method] Three DNA bar-coding genes ITS, ITS2 and rbcL were amplified from seven Xanthium species un-der the same PCR condition: PCR amplification was started with initial denaturation at 95 ℃ for 4 min, fol owed by 35 cycles of denaturation at 94 ℃ for 30 s, anneal-ing at 52 ℃ for 45 s, and extension at 72 ℃ for 45 s; the amplification was com-pleted by holding the reaction mixture at 72 ℃ for 10 min to al ow complete exten-sion of PCR, and the PCR products were stored at 4 ℃. [Result] Three DNA bar-coding genes ITS, ITS2 and rbcL were al amplified successful y. [Conclusion] This study indicates that PCR amplification conditions for DNA barcoding genes ITS, ITS2 and rbcL in plants may be consistent.
