中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
4期
697-699
,共3页
尪痹片%蛇床子素%二氢欧芹醇当归酸酯%高效液相色谱法
尪痺片%蛇床子素%二氫歐芹醇噹歸痠酯%高效液相色譜法
왕비편%사상자소%이경구근순당귀산지%고효액상색보법
Wangbi tablets%Osthole%Columbianadin%HPLC
目的：建立同时测定尪痹片中蛇床子素和二氢欧芹醇当归酸酯含量的高效液相色谱法。方法：色谱柱：Agilent XDB C18(150 m ×4.6 mm,5μm)；流动相：乙腈-水(49∶51)等度洗脱；流速：1.0 ml·min-1；检测波长：330 nm。结果：蛇床子素在0.060~0.597μg范围内线性关系良好(r=0.9999),平均回收率为99.12%；二氢欧芹醇当归酸酯在0.010~0.102μg范围内线性关系良好(r=0.9999),平均回收率为98.98%。结论：本方法简便、准确,重复性好,可用于尪痹片中上述两种成分的含量测定。
目的：建立同時測定尪痺片中蛇床子素和二氫歐芹醇噹歸痠酯含量的高效液相色譜法。方法：色譜柱：Agilent XDB C18(150 m ×4.6 mm,5μm)；流動相：乙腈-水(49∶51)等度洗脫；流速：1.0 ml·min-1；檢測波長：330 nm。結果：蛇床子素在0.060~0.597μg範圍內線性關繫良好(r=0.9999),平均迴收率為99.12%；二氫歐芹醇噹歸痠酯在0.010~0.102μg範圍內線性關繫良好(r=0.9999),平均迴收率為98.98%。結論：本方法簡便、準確,重複性好,可用于尪痺片中上述兩種成分的含量測定。
목적：건립동시측정왕비편중사상자소화이경구근순당귀산지함량적고효액상색보법。방법：색보주：Agilent XDB C18(150 m ×4.6 mm,5μm)；류동상：을정-수(49∶51)등도세탈；류속：1.0 ml·min-1；검측파장：330 nm。결과：사상자소재0.060~0.597μg범위내선성관계량호(r=0.9999),평균회수솔위99.12%；이경구근순당귀산지재0.010~0.102μg범위내선성관계량호(r=0.9999),평균회수솔위98.98%。결론：본방법간편、준학,중복성호,가용우왕비편중상술량충성분적함량측정。
Objective:To establish an HPLC method for the determination of osthole and columbianadin in Wangbi tablets. Meth-ods:The column was an Agilent XDB C18(150 mm ×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water(49∶51)with gradient elution. The flow rate was 1. 0 ml·min-1 and the detection wavelength was at 330 nm. Results: The calibration curve was linear within the range of 0. 060-0. 597 μg(r=0. 999 9) for osthole and 0. 010-0. 102 μg(r=0. 999 9) for columbianadin. The aver-age recovery was 99. 12% and 98. 98%, respectively. Conclusion:The method is simple, practicable, accurate and rapid. It can be applied in the content determination of osthole and columbianadin in Wangbi tablets.
