乳业科学与技术
乳業科學與技術
유업과학여기술
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
2012年
1期
8-10
,共3页
王佳丽%高学军%李庆章%黄建国%陆黎敏%乔彬%潘洪宝
王佳麗%高學軍%李慶章%黃建國%陸黎敏%喬彬%潘洪寶
왕가려%고학군%리경장%황건국%륙려민%교빈%반홍보
蛋氨酸%奶牛乳腺上皮细胞%乳糖%β-酪蛋白%甘油三酯%细胞增殖
蛋氨痠%奶牛乳腺上皮細胞%乳糖%β-酪蛋白%甘油三酯%細胞增殖
단안산%내우유선상피세포%유당%β-락단백%감유삼지%세포증식
methionine%dairy cow mammary epithelial cells%lactose%E-casein%triglyceride%cell proliferation
研究蛋氨酸与乳蛋白合成及乳腺泌乳能力的关系。建立体外培养的奶牛乳腺上皮细胞模型,采用高效液相色谱法、实时荧光定量PCR、细胞活力分析仪、甘油三酯试剂盒检测不同浓度蛋氨酸(0、0.2、0.4、0.8、1.2、1.6mmol/L)对体外培养24h的奶牛乳腺上皮细胞泌乳的影响。结果表明,蛋氨酸在1.2mmol/L时对体外培养的奶牛乳腺上皮细胞增殖的促进作用最明显,乳糖分泌量最高,β-酪蛋白mRNA表达量最高且甘油三酯合成最多(P〈0.01)。
研究蛋氨痠與乳蛋白閤成及乳腺泌乳能力的關繫。建立體外培養的奶牛乳腺上皮細胞模型,採用高效液相色譜法、實時熒光定量PCR、細胞活力分析儀、甘油三酯試劑盒檢測不同濃度蛋氨痠(0、0.2、0.4、0.8、1.2、1.6mmol/L)對體外培養24h的奶牛乳腺上皮細胞泌乳的影響。結果錶明,蛋氨痠在1.2mmol/L時對體外培養的奶牛乳腺上皮細胞增殖的促進作用最明顯,乳糖分泌量最高,β-酪蛋白mRNA錶達量最高且甘油三酯閤成最多(P〈0.01)。
연구단안산여유단백합성급유선비유능력적관계。건입체외배양적내우유선상피세포모형,채용고효액상색보법、실시형광정량PCR、세포활력분석의、감유삼지시제합검측불동농도단안산(0、0.2、0.4、0.8、1.2、1.6mmol/L)대체외배양24h적내우유선상피세포비유적영향。결과표명,단안산재1.2mmol/L시대체외배양적내우유선상피세포증식적촉진작용최명현,유당분비량최고,β-락단백mRNA표체량최고차감유삼지합성최다(P〈0.01)。
An in vitro model of dairy cow mammary epithelial cells (DCMECs) was developed and used to study the relationship between methionine and milk protein synthesis or the lactation ability of the mammary gland. After 24 hours of in vitro culture in the presence of various concentrations (0, 0.2, 0.4, 0.8, 1.2 mmol/L and 1.6 mmol/L) of methionine, the lactation ability of DCMECs was analyzed by high performance liquid Chromatography (HPLC), real-time quantitative PCR (RT-PCR), cell viability analyzer and triglyceride kit. It was found that methionine at 1.2 mmol/L had the most significant proliferation- promoting effect on DCMECs during the culture period. In addition, the highest amounts of lactose secretion, /3-casein mRNA expression and triglyceride synthesis were achieved.