CAJ | 학술논문

通过盐析透析、离了层析、疏水层析，对链霉菌属发酵液中的阿魏酸酯酶（FAE）进行分离纯化。并进行酶学性质研究。得出的阿魏酸酯酶的纯化倍数为2．67，回收率为55．8％，酶分子约为30kDa。其最适反应pH约为6．0，最适温度约为50℃，金属离子Cu^2＋和Zn^2＋对阿魏酸酯酶酶活力均有明显的抑制作用，而Mg^2＋、Fe^2＋、Ca^2＋、Na^＋和Mn^2＋对酶的活性均有一定的促进作用。
통과염석투석、리료층석、소수층석，대련매균속발효액중적아위산지매（FAE）진행분리순화。병진행매학성질연구。득출적아위산지매적순화배수위2．67，회수솔위55．8％，매분자약위30kDa。기최괄반응pH약위6．0，최괄온도약위50℃，금속리자Cu^2＋화Zn^2＋대아위산지매매활력균유명현적억제작용，이Mg^2＋、Fe^2＋、Ca^2＋、Na^＋화Mn^2＋대매적활성균유일정적촉진작용。
Ferulic acid esterase produced from the strain was purified by salted out, dialyzed, DEAE-sepharose, Phenyl-sepharose, enzyme specific activity was increased from 11.2 U/mg to 29.9 U/mg and activity recovery reached 55.8%. The molecular mass of this enzyme was estimated to be 30kDa by 0.8% SDS-PAGE. The ferulic acid esterase had an optimal pH value 6.0, and it was stable at pH value 4.0-6.0. Optimal （reaction） temperature of the ferulic acid esterase was 50 ℃, and it was very stable in 25℃-35℃. Metal ions Cu^2＋, Zn^2＋had negative effect on FAE, but Mg^2＋、Fe^2＋、Ca^2＋、Na^＋ Mn^2＋ have promotion effect on FAE.