CAJ | 학술논문

利用PCR扩增CPV-2bVP2蛋白的主要抗原表位区基因，并将该基因片段克隆到原核表达载体pGEX-6P-1上，通过诱导表达条件的摸索，实现了VP2蛋白主要抗原表位区在大肠杆菌中的高效表达，sDs—PAGE检测表达的融合蛋白主要以可溶形式存在，并利用亲和层析得到融合蛋白。
이용PCR확증CPV-2bVP2단백적주요항원표위구기인，병장해기인편단극륭도원핵표체재체pGEX-6P-1상，통과유도표체조건적모색，실현료VP2단백주요항원표위구재대장간균중적고효표체，sDs—PAGE검측표체적융합단백주요이가용형식존재，병이용친화층석득도융합단백。
To establish a prokaryotic expression system expressing major epitope domain of canine parvovirus VP2 pro- tein to serve the diagnosis of CPV, the fragment of CPV--2b VP2 gene was cloned from cloned veetor by PCR. Then, recombinant E. coli strain BL--21 was induced to express the fusion protein by IPTG. The expressed productions were detected by SDS--PAGE and western blot, and the results showed that the VP2 protein was efficiently expressed and the size of recombinant protein is 34KD according to being expected.