CAJ | 학술논문

以非洲山毛豆脱脂粉末为原料制备富含多肽的酶解液,并对其抗氧化活性进行了研究.以水解度和清除1,1-二苯基-2-三硝基苯肼（1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-（2,4,6-trinitrophenyl）hydrazyl,DP-PH）自由基为指标,选择最佳水解酶,在酶解时间、温度、pH、酶与底物比例、底物与水比例等单因素试验基础上,利用正交试验对最佳的酶解工艺条件进行优化,同时对酶解产物对DPPH.、.OH和O2-.的清除能力和抗脂质过氧化反应能力进行了研究.结果表明,碱性蛋白酶为最佳水解酶,其酶解最佳条件为：50℃pH 8.0、m（酶）∶m（底物）=2∶100、m（底物）∶m（水）=5∶100,酶解4 h;酶解液清除自由基能力大小顺序为DP-PH.〉.OH〉O2-.和抗脂质过氧化作用,最高清除率分别是91.58%、90.19%、43.56%和44.73%;当质量浓度大于0.6 g/L,酶解液抗脂质过氧化作用高于2,6-二叔丁基-4-甲基苯酚（Butylated hydroxytoluene,BHT）.
이비주산모두탈지분말위원료제비부함다태적매해액,병대기항양화활성진행료연구.이수해도화청제1,1-이분기-2-삼초기분정（1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-（2,4,6-trinitrophenyl）hydrazyl,DP-PH）자유기위지표,선택최가수해매,재매해시간、온도、pH、매여저물비례、저물여수비례등단인소시험기출상,이용정교시험대최가적매해공예조건진행우화,동시대매해산물대DPPH.、.OH화O2-.적청제능력화항지질과양화반응능력진행료연구.결과표명,감성단백매위최가수해매,기매해최가조건위：50℃pH 8.0、m（매）∶m（저물）=2∶100、m（저물）∶m（수）=5∶100,매해4 h;매해액청제자유기능력대소순서위DP-PH.〉.OH〉O2-.화항지질과양화작용,최고청제솔분별시91.58%、90.19%、43.56%화44.73%;당질량농도대우0.6 g/L,매해액항지질과양화작용고우2,6-이숙정기-4-갑기분분（Butylated hydroxytoluene,BHT）.
Defatted powder of Tephrosia vogelii was hydrolyzed to prepare hydrolysate rich in peptides,and its antioxidant activity was investigated by free radical scavenging assay.The best hydrolysis enzyme was selected on the degree of hydrolysis and the abilities to scavenge 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-（2,4,6-trinitrophenyl）hydrazyl（DPPH）free radical.Further,single factor and orthogonal experiments was employed to optimize pH,time,temperature,enzyme-to-substrate ratio and substrate-to-water ratio for maximizing polypeptide content.Besides,the scavenging activity of DPPH ·,· OH and O2-·and anti-lipid peroxidation were studied.The results showed that the most suitable enzyme was alcalase and the optimal hydrolysis conditions were 50 ℃ pH 8.0,m（substrate）∶ m（water） =5∶ 100,m（enzyme）∶ m（substrate） =2∶ 100,hydrolyzing 4 h.The effect on free radical scavenging capacity of Tephrosia vogelii polypeptides in the order was DPPH · 〉 · OH〉O2-· and liposome peroxide,the highest clearance rate were 91.58%,90.19%,43.56% and 44.73%,respectively.The polypeptide had strong anti-lipid peroxidation effect,higher than butylated hydroxytoluene（BHT）,when its mass concentration were greater than 0.6 g/L.