CAJ | 학술논문

以长白山林蛙中分离的嗜水气单胞菌为材料，对嗜水气单胞菌气溶素基因进行克隆、序列分析及基因表达等研究，为嗜水气单胞菌的分子生物学研究提供依据。通过实验成功克隆了气溶索基因，大小为1163bp，对其进行同源性检测，与基因库中DQ186611基因序列的同源性高达99％；构建原核表达重组质粒pGEX-Aer，在大肠杆菌BL21中获得表达，蛋白分子量约为69ku，以包涵体形式存在。
이장백산림와중분리적기수기단포균위재료，대기수기단포균기용소기인진행극륭、서렬분석급기인표체등연구，위기수기단포균적분자생물학연구제공의거。통과실험성공극륭료기용색기인，대소위1163bp，대기진행동원성검측，여기인고중DQ186611기인서렬적동원성고체99％；구건원핵표체중조질립pGEX-Aer，재대장간균BL21중획득표체，단백분자량약위69ku，이포함체형식존재。
Studied the Aerornonas hydrophila aerolysingene; s clone, sequence of gene; s analysis, pronucleus expression which separated from in experiment, providing the basis for the aeromonas hydrophila＇s molecular biology study. Succeed to clone the target gene sized 1 163 bp of Aer from Changbai mountain Raha,countered the recombinant through sequencing and obtain 99; homology between the Aer gene of recombinant and DQ186611 published from the GenBank. And found the peak output of vector pGEX-Aer in this experiment,which can expressed in E. eoli BL21. The fusion protein has a molecular weight of 69 ku , most of which existed in the mode of inclusion body.