CAJ | 학술논문

在水温25.2～28.2℃下,将半滑舌鳎（Cynoglossus semilaevis）（体质量120.65±1.57g）饲养于底面积为0.75m2的圆形水族内（30尾/箱）,用黑塑料布遮光,研究了4种光照周期（0L/24D,6L/18D,18L/6D,24L/0D）对生长和抗氧化指标的影响。28 d的饲养结果表明：光照周期为6L/18D时,饲料系数最低、增重率和净增重最大;光照周期为18L/6D时,血清中超氧化物歧化酶（SOD）活力最高,而在24L/0D光照周期下,肝脏中SOD比活力最高,在四种光照周期下,脾脏、肾脏SOD比活力差异不显著（P〉0.05）;光照周期为6L/18D时,血清过氧化氢酶（CAT）活力最高,6L/18D组鱼脾脏中CAT比活力显著高于其他组（P〈0.05）;肝脏、肾脏中CAT的比活力差异不显著（P〉0.05）;肝脏的还原型谷胱甘肽（GSH）含量均显著高于血清、脾脏和肾脏,6L/18D组鱼肝脏中（GSH含量显著高于0L/24D组和24L/0D组（P〈0.05）;6L/18D组血清中丙二醛（MDA）含量显著低于其他组（P〈0.05）,肝脏中0L/24D组MDA含量最低,18L/6D组MDA含量显著高于0L/24D组（P〈0.05）,6L/18D组鱼脾脏、肾脏中MDA含量最低。光照周期为6L/18D时最适合半滑舌鳎生长。
재수온25.2～28.2℃하,장반활설탑（Cynoglossus semilaevis）（체질량120.65±1.57g）사양우저면적위0.75m2적원형수족내（30미/상）,용흑소료포차광,연구료4충광조주기（0L/24D,6L/18D,18L/6D,24L/0D）대생장화항양화지표적영향。28 d적사양결과표명：광조주기위6L/18D시,사료계수최저、증중솔화정증중최대;광조주기위18L/6D시,혈청중초양화물기화매（SOD）활력최고,이재24L/0D광조주기하,간장중SOD비활력최고,재사충광조주기하,비장、신장SOD비활력차이불현저（P〉0.05）;광조주기위6L/18D시,혈청과양화경매（CAT）활력최고,6L/18D조어비장중CAT비활력현저고우기타조（P〈0.05）;간장、신장중CAT적비활력차이불현저（P〉0.05）;간장적환원형곡광감태（GSH）함량균현저고우혈청、비장화신장,6L/18D조어간장중（GSH함량현저고우0L/24D조화24L/0D조（P〈0.05）;6L/18D조혈청중병이철（MDA）함량현저저우기타조（P〈0.05）,간장중0L/24D조MDA함량최저,18L/6D조MDA함량현저고우0L/24D조（P〈0.05）,6L/18D조어비장、신장중MDA함량최저。광조주기위6L/18D시최괄합반활설탑생장。
The half-smooth tongue-sole （Cynoglossus semilaevis Gu ntuer） with body weight of （ 120.65± 1.57g）were reared in circu- lar 0.75 m： bottom surface area tanks at a rate of 30 fish/tank under photoperiod of 0L：24D, 6L： 18D, 18L：6D and 24L：0D for 28d at water temperature of 25.2 -8.2℃ to evaluate effects of photoperiod on growth and antioxidant index.. The results showed that the minimal food conversion ratio, and the maximal weight gain rate and net weight gain were observed in the fish in photoperiod of 6L/18D. Under the photoperiod of 24L/0D, the maximum specific activity of superoxide dismutase （SOD） was found in the liver of the fish while there were no significant differences in SOD activity in the spleen and kidney （P〉0.05）. The maximal specific activity of catalase （CAT） was found in the serum in photoperiod of 6L/18D, significant higher in spleen in 6 L/18D than that in other groups （P〈0.05）. There were no significant differences in CAT activity in liver and kidney under four photoperiods （P〉0.05）. There was sig- nificant higher glutathione （GSH） content in the liver than that in the serum, spleen and kidney （P〈0.05）. The GSH content in the liv- er was found significantly higher in 6L/18D than that in OL/24D and 24L/0D （P〈0.05）while the malonaldehyde （MDA）content in serum significantly lower than that in other groups （P〈0.05）. The MDA in the liver was found to be the minimum in 0L/24D, signifi- cant higher in 18L/6D than that in 0L/24D （P〈0.05）. The minimum MDA activity in spleen and kidney was observed in 6L/18D, indicating that 6L/18D is the optimum photoperiod for growth of the fish.