水产学杂志
水產學雜誌
수산학잡지
CHINESE JOURNAL OF FISHERIES
2012年
2期
7-10,14
,共5页
回交%荷包红鲤%鲤%染色体核型
迴交%荷包紅鯉%鯉%染色體覈型
회교%하포홍리%리%염색체핵형
backcross%Purse Red Carp%Common Carp%chromosome karyotype
在23~25℃下,给平均体质量104.2g和106.8g的回交鲤(鲤鲫杂交♀×鲤♂)和回交荷包红鲤(鲤鲫杂交♀×荷包红鲤♂)胸腔注射植物凝集素(PHA)和秋水仙素,然后观察对比了染色体核型,探讨回交后代的染色体遗传。结果表明,两种鲤鲫杂交回交子代染色体组均由150条染色体组成,按着丝粒位置染色体组型可分为四组,每个染色体小组均由三条同源染色体组成。其中,回交鲤染色体数目为3n=150,染色体臂数NF=234,其核型公式为:3n=60m+24sm+36st+30t;回交荷包红鲤的染色体数目为3n=150,染色体臂数NF=252,其核型公式为:3n=66m+36sm+18st+30t。
在23~25℃下,給平均體質量104.2g和106.8g的迴交鯉(鯉鯽雜交♀×鯉♂)和迴交荷包紅鯉(鯉鯽雜交♀×荷包紅鯉♂)胸腔註射植物凝集素(PHA)和鞦水仙素,然後觀察對比瞭染色體覈型,探討迴交後代的染色體遺傳。結果錶明,兩種鯉鯽雜交迴交子代染色體組均由150條染色體組成,按著絲粒位置染色體組型可分為四組,每箇染色體小組均由三條同源染色體組成。其中,迴交鯉染色體數目為3n=150,染色體臂數NF=234,其覈型公式為:3n=60m+24sm+36st+30t;迴交荷包紅鯉的染色體數目為3n=150,染色體臂數NF=252,其覈型公式為:3n=66m+36sm+18st+30t。
재23~25℃하,급평균체질량104.2g화106.8g적회교리(리즉잡교♀×리♂)화회교하포홍리(리즉잡교♀×하포홍리♂)흉강주사식물응집소(PHA)화추수선소,연후관찰대비료염색체핵형,탐토회교후대적염색체유전。결과표명,량충리즉잡교회교자대염색체조균유150조염색체조성,안착사립위치염색체조형가분위사조,매개염색체소조균유삼조동원염색체조성。기중,회교리염색체수목위3n=150,염색체비수NF=234,기핵형공식위:3n=60m+24sm+36st+30t;회교하포홍리적염색체수목위3n=150,염색체비수NF=252,기핵형공식위:3n=66m+36sm+18st+30t。
The chromosome numbers and karyotypes were observed, and compared in offsprings of backcross of purse red carp (hybrids of common carp Cyprinus carpio and crucian carp ♂ Carassius auratus × purse red carp 8 Cyprinus carpio var. wuyuanensis) by intraperitoneal injection of phytohemagglutinin (PHA) and colchicines to evaluate the chromosome genetics of the offsprings. The results showed that the chromosome numbers of the two backcross offsprings of common carp and crucian carp were both 150, and divided into four chromosome groups of each being consisted of 3 homologous chromosomes, depending upon the position of centromere. In the backcross offsprings of the purse red carp, chromosome numbers and karyotypic formula were expressed to be 3n = 150, NF=252 and 3n=66m+36sm+18st+30t. The backcross offsprings of common carp, chromosome numbers and karyotypic formula were found to be 3n=150, NF=234 and 3n =60m+ 24sm+ 36st+30t.