CAJ | 학술논문

鲫（Carassius auratus）遗传多样性丰富,具多种不同倍性的亚种,是研究鱼类进化的独特材料。本研究利用79个SSR标记和22个EST-SSR标记检测了鲫自交F2代的181尾个体的基因型,对体重、体长、体高和体厚4个生长性状进行了单标记回归分析。Permutation检验（10000次）结果显示：有22个标记分别与体重、体长、体高和体厚具有显著相关性（P〈0.05）,其中,HLJYJ15和HLJYJ244与4个性状均呈极显著相关（P〈0.01）。本研究筛选的101个微卫星标记丰富了鲫分子标记数量,可用于鲫连锁图谱的构建和QTL定位研究;获得了4个与鲫性状相关的标记及基因型,为鲫的分子标记辅助育种（MAS）和生长性状的遗传机制研究提供了工具。
즉（Carassius auratus）유전다양성봉부,구다충불동배성적아충,시연구어류진화적독특재료。본연구이용79개SSR표기화22개EST-SSR표기검측료즉자교F2대적181미개체적기인형,대체중、체장、체고화체후4개생장성상진행료단표기회귀분석。Permutation검험（10000차）결과현시：유22개표기분별여체중、체장、체고화체후구유현저상관성（P〈0.05）,기중,HLJYJ15화HLJYJ244여4개성상균정겁현저상관（P〈0.01）。본연구사선적101개미위성표기봉부료즉분자표기수량,가용우즉련쇄도보적구건화QTL정위연구;획득료4개여즉성상상관적표기급기인형,위즉적분자표기보조육충（MAS）화생장성상적유전궤제연구제공료공구。
Crucian carp Carassius auratus is rich in genetic diversity, and becomes a good candidate for research of molecular markers or genes associated with those growth traits. In the current study, 79 SSR and 22 EST-SSR markers were chosen to analysis the genom- ic of 181 progenies from a crucian carp F2 family. A GLM （General Linear Model） procedure was used to analyze the correlation be- tween markers and four growth traits including body weight, body length, body depth and body width. The significance testing by Per- mutation test（ 10 000 replicates）, 22 makers were shown to fit the correlation from four growth traits （P〈0.05）, very significant corre- lation （P〈0.01） for HLJYJ15 and HLYJ244. Markers and gene types related to the four growth traits were obtained from this research, which gives effective basis to the study of crucian carp marker-assisted breeding and genetic mechanism of growth traits.