花生学报
花生學報
화생학보
PEANUT SCIENCE AND TECHNOLOGY
2012年
2期
8-12
,共5页
王洁%李双铃%王辉%石延茂%任艳%何国浩%袁美
王潔%李雙鈴%王輝%石延茂%任豔%何國浩%袁美
왕길%리쌍령%왕휘%석연무%임염%하국호%원미
花生%杂种鉴定%分子标记%转座子%AhMITE1%PCR
花生%雜種鑒定%分子標記%轉座子%AhMITE1%PCR
화생%잡충감정%분자표기%전좌자%AhMITE1%PCR
Arachis hypogaea L.%hybrid identification%molecular marker%transposable element%AhMITE1%PCR
对杂交后代进行杂种的真实性鉴定是杂交育种成功的前提。AhMITE1转座子标记是一类基于PCR的分子标记,其在花生中扩增的产物片段差异大,多态性强,采用琼脂糖凝胶电泳即可区分。本研究用7对多态性转座子引物对6个花生组合的166个F1单株进行了真实性鉴定。根据杂种植株含有父母本带型为依据共鉴定出了77个单株为真杂种,真杂种植株数占植株总数的比率为44.86%;收获时根据田间表型鉴定出了79个单株为真杂种,真杂种比率为47.59%。
對雜交後代進行雜種的真實性鑒定是雜交育種成功的前提。AhMITE1轉座子標記是一類基于PCR的分子標記,其在花生中擴增的產物片段差異大,多態性彊,採用瓊脂糖凝膠電泳即可區分。本研究用7對多態性轉座子引物對6箇花生組閤的166箇F1單株進行瞭真實性鑒定。根據雜種植株含有父母本帶型為依據共鑒定齣瞭77箇單株為真雜種,真雜種植株數佔植株總數的比率為44.86%;收穫時根據田間錶型鑒定齣瞭79箇單株為真雜種,真雜種比率為47.59%。
대잡교후대진행잡충적진실성감정시잡교육충성공적전제。AhMITE1전좌자표기시일류기우PCR적분자표기,기재화생중확증적산물편단차이대,다태성강,채용경지당응효전영즉가구분。본연구용7대다태성전좌자인물대6개화생조합적166개F1단주진행료진실성감정。근거잡충식주함유부모본대형위의거공감정출료77개단주위진잡충,진잡충식주수점식주총수적비솔위44.86%;수획시근거전간표형감정출료79개단주위진잡충,진잡충비솔위47.59%。
Accurate identification of F1 hybrid is critical to hybridization breeding in peanut. MITE transposable marker is a type.of PCR marker with high polymorphism and easy detection by agarose gel electrophoresis. 7 pairs of polymorphic primers were used to amplify 166 F1 individual plants from 6 crosses. Out of those plants, 77 plants were identified as true hybrids, accounting for 44,86%. Based on phenotype and agronomic traits, 79 plants were identified as true hybrids, accounting for 47.59%.
