CAJ | 학술논문

用构建好的重组质粒转化枯草芽孢杆菌（Bacillus subtilis）WB600感受态细胞，进行不同时间预培养后，涂布于抗生素平板培养，研究一种更高效的转化方法。用载体与外源片段的连接产物转化WB600感受态细胞，进行最佳预培养时间培养后，涂布于抗生素平板培养，培养得到的转化子用PCR快速验证和双酶切鉴定，研究一种转化子的快速验证方法。
용구건호적중조질립전화고초아포간균（Bacillus subtilis）WB600감수태세포，진행불동시간예배양후，도포우항생소평판배양，연구일충경고효적전화방법。용재체여외원편단적련접산물전화WB600감수태세포，진행최가예배양시간배양후，도포우항생소평판배양，배양득도적전화자용PCR쾌속험증화쌍매절감정，연구일충전화자적쾌속험증방법。
In order to study an effective method for transformation, Bacillus subtilis WB600 competent cells, transformed with pre-built recombinant plasmids, were precultured with different period of time and smeared to the plates containing antibiotics. In order to construct a fast and effective method for transformants verification, WB600 competent cells were transformed with vector and foreign DNA, preincubated with the optimal time, and smeared to the plates containing antibiotics. The transformants were verified with PCR and double restriction enzyme digestion.