陕西师范大学学报:自然科学版
陝西師範大學學報:自然科學版
협서사범대학학보:자연과학판
Journal of Shaanxi Normal University: Nat Sci Ed
2012年
3期
62-66
,共5页
欧阳霞辉%李春鸣%魏锁成%侯兰新%奚耕思
歐暘霞輝%李春鳴%魏鎖成%侯蘭新%奚耕思
구양하휘%리춘명%위쇄성%후란신%해경사
拟黑多刺蚁%pvERR%原位杂交%昆虫脑部
擬黑多刺蟻%pvERR%原位雜交%昆蟲腦部
의흑다자의%pvERR%원위잡교%곤충뇌부
Polyrhachis vicina%pvERR%in situ hybridization%insect brain
采用原位杂交方法对不同品级拟黑多刺蚁(Polyrhachis vicina)脑部雌激素相关受体pvERRmRNA的表达进行了检测.结果表明,pvERRmRNA在不同品级的蕈形体、附叶、视叶、中脑、食道下神经节和球状细胞中都有表达.蚁后蕈形体和球状细胞中pvERRmRNA的阳性表达最为明显;工蚁中脑pvERR mRNA的阳性反应强度明显高于蚁后和雄蚁;pvERR mRNA在雄蚁视叶中有较强的表达.pvERR mRNA在不同品级的拟黑多刺蚁脑部的表达差异可能与它们各自在蚁群中的功能和行为有关.
採用原位雜交方法對不同品級擬黑多刺蟻(Polyrhachis vicina)腦部雌激素相關受體pvERRmRNA的錶達進行瞭檢測.結果錶明,pvERRmRNA在不同品級的蕈形體、附葉、視葉、中腦、食道下神經節和毬狀細胞中都有錶達.蟻後蕈形體和毬狀細胞中pvERRmRNA的暘性錶達最為明顯;工蟻中腦pvERR mRNA的暘性反應彊度明顯高于蟻後和雄蟻;pvERR mRNA在雄蟻視葉中有較彊的錶達.pvERR mRNA在不同品級的擬黑多刺蟻腦部的錶達差異可能與它們各自在蟻群中的功能和行為有關.
채용원위잡교방법대불동품급의흑다자의(Polyrhachis vicina)뇌부자격소상관수체pvERRmRNA적표체진행료검측.결과표명,pvERRmRNA재불동품급적심형체、부협、시협、중뇌、식도하신경절화구상세포중도유표체.의후심형체화구상세포중pvERRmRNA적양성표체최위명현;공의중뇌pvERR mRNA적양성반응강도명현고우의후화웅의;pvERR mRNA재웅의시협중유교강적표체.pvERR mRNA재불동품급적의흑다자의뇌부적표체차이가능여타문각자재의군중적공능화행위유관.
The expression of pvERR mRNA in the brain of different caste of Polyrhachis vicina was detected by in situ hybridization method. The results showed that pvERR mRNA has broad expression pattern in the brain of different castes, which can be detected in mushroom body, accessory lobe, optic lobes, deutocerebrum, suboesophageal ganglion and kenyon cells. Strongly positive expression was detected in mushroom body and kenyon cells in brain of queen ant. Compared with queen ant, the expression degree of pvERR mRNA is stronger in the deutocerebrum of worker ant, but other parts are equivalent. Compared with queen and worker ants, the degree of pvERR expression is weaker in mushroom body, accessory lobe and suboesophageal ganglion of male ants, but stronger expression was found in optic lobes.