CAJ | 학술논문

采用单因素实验和L9（34）正交试验，研究纤维素酶酶解时间、酶解温度、酶解pH、酶加量对多糖得率的影响。利用还原糖测定仪测定经酸水解的多糖。结果表明，酶的反应温度和酶解pH值是提取玫瑰多糖的主要因素。最佳工艺方案为：酶反应温度50℃，酶反应pH值为4．6，酶反应时间150min，酶加量为4．5％。酶反应完后以料液比1：30在100℃下提取5h，在此工艺条件下，提取液中玫瑰多糖的得率为11．1％，可溶性多糖提取率为4．49％。
채용단인소실험화L9（34）정교시험，연구섬유소매매해시간、매해온도、매해pH、매가량대다당득솔적영향。이용환원당측정의측정경산수해적다당。결과표명，매적반응온도화매해pH치시제취매괴다당적주요인소。최가공예방안위：매반응온도50℃，매반응pH치위4．6，매반응시간150min，매가량위4．5％。매반응완후이료액비1：30재100℃하제취5h，재차공예조건하，제취액중매괴다당적득솔위11．1％，가용성다당제취솔위4．49％。
We employ single factor test and orthogonal test design method L9 （34） to analyze such influence factors as enzymolysis temperature, enzymolysis pH, enzymolysis time and cellulose amount on the yield rate of the polysaccharide. We also use reducing sugar analyzer to determine the amount of polysaccharide. Results show that temperature and pH are two dominant factors. The optimal enzymolysis condition is temperature of 50 ℃, pH of 4.6, 150 min and cellulose amount of 4.5%. The extract ratio of rose dregs polysaccharide is 11.1% and the yield rate of soluble polysaccharide is 4.49% in condition of the ratio of rose dreas and water of 1： 30. temoerature of 100 ℃ and extract time of 5 h.