CAJ | 학술논문

对泸型大曲中根霉菌株固态发酵产糖化酶的条件进行了研究。以糖化酶活力为评价指标，设计单因素实验，研究培养基水分含量、培养时间、培养温度对根霉产糖化酶的影响，在此基础上，利用Box—BenhnkenDesign的方法，对根霉菌株固态发酵产糖化酶的条件进行优化，结果显示泸型大曲中根霉菌株固态发酵产糖化酶的优化条件为培养温度29℃，培养基水分含量53％，培养时间164h，糖化酶的酶活力为2194．44U／g。
대로형대곡중근매균주고태발효산당화매적조건진행료연구。이당화매활력위평개지표，설계단인소실험，연구배양기수분함량、배양시간、배양온도대근매산당화매적영향，재차기출상，이용Box—BenhnkenDesign적방법，대근매균주고태발효산당화매적조건진행우화，결과현시로형대곡중근매균주고태발효산당화매적우화조건위배양온도29℃，배양기수분함량53％，배양시간164h，당화매적매활력위2194．44U／g。
The conditions of glucoamylase production of the Rhizopus oryzae in Luzhou - flavor Daqu by solid state fer- mentation are studied. By single factor experiment and saccharifying enzymic activityas as an assessing index, the influence on saccharifying enzymic aetivityas of the water content in culture medium, the culture time, the culture temperature are stud- ied. According to the Box-Benhnken design, the conditions of glucoamylase production of the Rhizopus oryzae are optimized by solid state fermentation. The results show that the optimum conditions of glucoamylase production of the Rhizopus oryzae in Luzhou-flavor Daqu by solid state fermentation are the culture temperature 29℃, the water content in culture medium 53%, culture time 164h, glucoamylase activity was 2 194.44U/g.