CAJ | 학술논문

目的：建立激光捕获显微切割技术与低体积扩增技术联用，进行冰冻切片上胚胎与母体成分DNA的分离检验技术。方法：制备早期流产组织冰冻切片，通过激光捕获显微切割胚胎绒毛细胞，设置10，20，30个细胞数目梯度，比较各组胚胎成分DNA的STR分型的检出率、等位基因丢失率和非特异性扩增情况。最后进行实际样本应用。结果：捕获10个胚胎绒毛细胞即可获得Identifier试剂完整的STR分型。冰冻切片捕获30个细胞数目组的检出率较高，等位基因丢失率和非特异性扩增率较低。结论：激光捕获显微切割技术联用低体积扩增技术可应用于胚胎与母体成分DNA的精确分离和检验。
목적：건립격광포획현미절할기술여저체적확증기술련용，진행빙동절편상배태여모체성분DNA적분리검험기술。방법：제비조기유산조직빙동절편，통과격광포획현미절할배태융모세포，설치10，20，30개세포수목제도，비교각조배태성분DNA적STR분형적검출솔、등위기인주실솔화비특이성확증정황。최후진행실제양본응용。결과：포획10개배태융모세포즉가획득Identifier시제완정적STR분형。빙동절편포획30개세포수목조적검출솔교고，등위기인주실솔화비특이성확증솔교저。결론：격광포획현미절할기술련용저체적확증기술가응용우배태여모체성분DNA적정학분리화검험。
Objective： To establish a technology platform, using single- cell separation technique and LCM to realize accurate separation and testing fetal and maternal tissue in mixture of samples~ Method： Frozen section slides were prepared, setting the number of gradient cells 10,20, 30 , then, the artificial alleles, detection rate and allelie dropout rate of each group were compared. The technology was applied into an actual case. Result：Capturing 10 cells of villus can obtain the fetal STR - DNA type. The detection rate of 30 cells is high. Artificial alleles and allelie dropout rate are low. Conclusion： Single - cell separation technique combined with LCM can be applied to accurately separate and test mixture of fetal and maternal tissue, so as to improve evidence value of mixed biological samoles.