农业研究与应用
農業研究與應用
농업연구여응용
GUANGXI TROPICAL AGRICULTURE
2012年
4期
16-19
,共4页
陈雪凤%韦香%陈思名%杨芳%李永明%刘明福
陳雪鳳%韋香%陳思名%楊芳%李永明%劉明福
진설봉%위향%진사명%양방%리영명%류명복
茶薪菇%生物学特性%鸡粪栽培
茶薪菇%生物學特性%鷄糞栽培
다신고%생물학특성%계분재배
Agrocybe aegerita Sp.%biological characteristics%cultivation with poultry dung
取野生茶薪菇茵柄与茵盖交界处组织块分离成功率高达75%,分离获得的纯化茵株命名为“茶薪菇玉微1号”。该菌株主要生物学特性为:茵丝最适宜生长温度为25-30℃;最适宜生长pH值为6~7;在4g蛋白胨+PDA加富、20g麦麸+PDA加富母种培养基上茵丝生长较好。利用15%的鸡粪替代麦麸栽培茶薪菇生物转化率达85.6%。
取野生茶薪菇茵柄與茵蓋交界處組織塊分離成功率高達75%,分離穫得的純化茵株命名為“茶薪菇玉微1號”。該菌株主要生物學特性為:茵絲最適宜生長溫度為25-30℃;最適宜生長pH值為6~7;在4g蛋白胨+PDA加富、20g麥麩+PDA加富母種培養基上茵絲生長較好。利用15%的鷄糞替代麥麩栽培茶薪菇生物轉化率達85.6%。
취야생다신고인병여인개교계처조직괴분리성공솔고체75%,분리획득적순화인주명명위“다신고옥미1호”。해균주주요생물학특성위:인사최괄의생장온도위25-30℃;최괄의생장pH치위6~7;재4g단백동+PDA가부、20g맥부+PDA가부모충배양기상인사생장교호。이용15%적계분체대맥부재배다신고생물전화솔체85.6%。
An Agrocybe aegerita Sp. strain named "Agrocybe Yuwei 1" was isolated and purified by tissue isolation method. The isolation rate was 75% when isolated from the tissue of the connection of the stipe and the pileus. The observation on biological characteristics revealed that the most suitable temperature for the mycelia growth was 25 -30℃ ; the optimum pH was pH6-7; the mycelia grew well in the medium of 4g peptone + PDA enrichment or 20g wheat bran + PDA enrichment. The experiment results showed that the biological efficiency was 85% when wheat bran was substituted with 15% poultry dung as the culture medium.