中华内分泌外科杂志
中華內分泌外科雜誌
중화내분비외과잡지
CHINESE JOURNAL OF ENDOCRINE SURGERY
2013年
2期
141-143
,共3页
糖耐量减低%α细胞%超微结构
糖耐量減低%α細胞%超微結構
당내량감저%α세포%초미결구
Impaired glucose tolerance%alpha cells%Ultrastructure
目的 探讨糖耐量减低阶段大鼠胰岛α细胞超微结构改变.方法 16只Wistar大鼠,随机分为糖耐量正常组(NGT组,n=8)和糖耐量减低组(IGT组,n=8).NGT组给予常规饲料,IGT组给予高糖高脂饲料.12周时行口服葡萄糖耐量(OGTT)试验,以7.8 mmol/L≤2 h PG＜ 11.1 mmol/L并持续1周以上为IGT造模成功.取2组大鼠胰尾,在透射电镜下观察d细胞的超微结构.结果 NGT组α细胞内含带晕环的分泌颗粒,且颗粒饱满、较大,内有电子密度高的致密芯,芯为圆形,核染色质及内容物分布均匀,细胞内线粒体、内质网排列紧密、整齐.IGT组胰岛α细胞分泌颗粒增多,致密芯与界膜间的间隙变窄,线粒体增多、变形、排列紊乱,粗面内质网增多.结论 胰岛α细胞超微结构改变和功能异常与糖耐量减低有关.
目的 探討糖耐量減低階段大鼠胰島α細胞超微結構改變.方法 16隻Wistar大鼠,隨機分為糖耐量正常組(NGT組,n=8)和糖耐量減低組(IGT組,n=8).NGT組給予常規飼料,IGT組給予高糖高脂飼料.12週時行口服葡萄糖耐量(OGTT)試驗,以7.8 mmol/L≤2 h PG＜ 11.1 mmol/L併持續1週以上為IGT造模成功.取2組大鼠胰尾,在透射電鏡下觀察d細胞的超微結構.結果 NGT組α細胞內含帶暈環的分泌顆粒,且顆粒飽滿、較大,內有電子密度高的緻密芯,芯為圓形,覈染色質及內容物分佈均勻,細胞內線粒體、內質網排列緊密、整齊.IGT組胰島α細胞分泌顆粒增多,緻密芯與界膜間的間隙變窄,線粒體增多、變形、排列紊亂,粗麵內質網增多.結論 胰島α細胞超微結構改變和功能異常與糖耐量減低有關.
목적 탐토당내량감저계단대서이도α세포초미결구개변.방법 16지Wistar대서,수궤분위당내량정상조(NGT조,n=8)화당내량감저조(IGT조,n=8).NGT조급여상규사료,IGT조급여고당고지사료.12주시행구복포도당내량(OGTT)시험,이7.8 mmol/L≤2 h PG＜ 11.1 mmol/L병지속1주이상위IGT조모성공.취2조대서이미,재투사전경하관찰d세포적초미결구.결과 NGT조α세포내함대훈배적분비과립,차과립포만、교대,내유전자밀도고적치밀심,심위원형,핵염색질급내용물분포균균,세포내선립체、내질망배렬긴밀、정제.IGT조이도α세포분비과립증다,치밀심여계막간적간극변착,선립체증다、변형、배렬문란,조면내질망증다.결론 이도α세포초미결구개변화공능이상여당내량감저유관.
Objective To investigate the ultrastructure changes of pancreatic islets alpha cells in rats with impaired glucose tolerance.Methods 16 Wistar rats were randomly assigned into normal glucose tolerance group(NGT group,n =8)and impaired glucose tolerance group(IGT group,n =8).NGT group were fed with routine diet and IGT-group were fed with high-sugar high-fat diet.At the 12th week,IGT models were considered successful if the level of 2-hour peripheral blood glucose(2 h PG)was between 7.8 and 11.1 mmol/L in oral glucose tolerance test and continued for more than a week.The pancreas tail of animals in the NGT group and IGT group rats was removed for ultrastructure observation.Alpha cells were observed under transmission electron microscope.Results The big-plump secretory granule with aureole appeared in pancreatic islets alpha cells of NGT group,and it contained a round dense core.The nuclear chromatin and contents were evenly distributed.Mitochondria and en-doplasmic reticulum arranged tightly and orderly.Compared with NGT group,the quantities of secretory granule increased significantly in IGT group.The gap between dense core and membrane was narrower.The quantities of mitochondria and rough endoplasmic reticulum increased significantly.There was no significant change for the structure of the other cell organelle.Conclusion The pathogenesis of IGT is associated with ultrastructural changes and dysfunction of pancreas islets alpha cells.
