CAJ | 학술논문

目的探讨BD针在重症急性胰腺炎急性肺损伤研究中大鼠模型建立的改良应用.方法健康成年雄性SD大鼠40只,随机分为模型组(n=30)、假手术组(n=10),应用BD静脉留置针经十二指肠乳头逆行胰胆管注入4%牛磺胆酸(0.1 mL/ks),建立重症急性胰腺炎急性肺损伤大鼠模型.模型组在建立后3 h、6 h、12 h检测大鼠血淀粉酶、血气分析、腹水量、肺含水量,光镜下进行胰腺和肺组织病变程度评分.假手术组在术后12 h检测上述相关指标.结果模型组较假手术组血淀粉酶、腹水量、肺含水率、PaCO2、胰腺和肺组织学评分明显升高(P＜0.05).同时PaO2,氧合指数明显降低(P＜0.05).结论应用BD静脉留置针能有效制备简便、易复制、稳定性好的重症急性胰腺炎急性肺损伤大鼠模型.
목적 탐토BD침재중증급성이선염급성폐손상연구중대서모형건립적개량응용.방법 건강성년웅성SD대서40지,수궤분위모형조(n=30)、가수술조(n=10),응용BD정맥류치침경십이지장유두역행이담관주입4%우광담산(0.1 mL/ks),건립중증급성이선염급성폐손상대서모형.모형조재건립후3 h、6 h、12 h검측대서혈정분매、혈기분석、복수량、폐함수량,광경하진행이선화폐조직병변정도평분.가수술조재술후12 h검측상술상관지표.결과 모형조교가수술조혈정분매、복수량、폐함수솔、PaCO2、이선화폐조직학평분명현승고(P＜0.05).동시PaO2,양합지수명현강저(P＜0.05).결론 응용BD정맥류치침능유효제비간편、역복제、은정성호적중증급성이선염급성폐손상대서모형.
Objective To investigate the improvement of BD intravenous catheters used in inducing the model of acute lung injury with severe acute pancreatitis in rats.Methods Total 40 healthy adult male SD rats were randomly assigned to 2 groups,SO group(n=10),SAP group(n=30),4% tauroeholate(0.1ml/kg)was injected via retrograde biliopancreatic ducts by using BD intravenous catheters via duodenal papilla.The serum amylase,blood gas analysis,ascites volume,lung water content and the pathological score of pancreas and lung structure under light microscope were recorded when the SAP model were induced in 3 h,6 h and 12 h.The relative data mentioned as above also detected in SO group after 12 h.Results The score of the SAP group was significantly higher than the SO group in blood amylase,ascites volume,lung water content,PaCO2,pancreas and lung histology(P＜0.05).Meanwhile,PaO2,oxygenation index were decreased significantly(P＜0.05).Conclusion It is efficient to establish simple,reproducible and stabile rat model of acute lung injury with severe acute pancreatitis by using BD intravenous catheters.