CAJ | 학술논문

　　里氏木霉菌Trichoderma reesei QM 6a蛋白激发子基因xyn2及绿木霉菌蛋白激发子基因sm1通过调节植物乙烯、茉莉酸等信号途径激发其诱导抗性，提高其免疫力。本研究根据天蓝色链霉菌偏爱密码子人工合成了这两个基因，将其单价连接到链霉菌表达载体pIB139中。通过酶切鉴定的阳性质粒pIB139-xyn2(sm1)转化入ET12567(pUZ8002)中间菌株，利用接合转移技术定点整合到利迪链霉菌A01基因组中，采用PCR技术鉴定工程菌株A01-xyn2(sm1)。随后构建A01-xyn2+sm1双价链霉菌工程菌株并验证。实验结果表明：利用接合转移技术已成功获得含 xyn2，sm1，xyn2＋sm1的链霉菌工程菌株，将实现抗生与诱抗的协同作用，提高防治植物病害的效果。
　　리씨목매균Trichoderma reesei QM 6a단백격발자기인xyn2급록목매균단백격발자기인sm1통과조절식물을희、말리산등신호도경격발기유도항성，제고기면역력。본연구근거천람색련매균편애밀마자인공합성료저량개기인，장기단개련접도련매균표체재체pIB139중。통과매절감정적양성질립pIB139-xyn2(sm1)전화입ET12567(pUZ8002)중간균주，이용접합전이기술정점정합도리적련매균A01기인조중，채용PCR기술감정공정균주A01-xyn2(sm1)。수후구건A01-xyn2+sm1쌍개련매균공정균주병험증。실험결과표명：이용접합전이기술이성공획득함 xyn2，sm1，xyn2＋sm1적련매균공정균주，장실현항생여유항적협동작용，제고방치식물병해적효과。
Trichoderma reesei QM 6a is characterized with a high level of XYN2 elicitor, which induces ethylene signaling pathway in plants, while sm1 elicitor produced by Trichoderma virens Gv29-8 is proved to induce jasmonic acid and ethylene signaling pathway. Both the elicitors could enhance the resistance of pathogens in plants. In this study, in order to improve the control effect of fungal pathogens by synergy of natamycin and elicitors, xyn2 and sm1 genes were synthetized according to the codon preference of Streptomyces coelicolor, linked into pIB139 expression vector and identified via enzyme digestion. The positive plasmid pIB139-xyn2 (sm1) was transformed to ET12567 (pUZ8002) intermediate strain and further into the genome of S. lydicus A01 by conjugal-transformed method, respectively. Then, for higher induce resistanc, S. lydicus A01 transformant with both xyn2 and sm1 was constructed. The results showed that:The three conjugal-transformants (A01-xyn2,A01-sm1,A01-xyn2+sm1 ) were constructed successfully, and they will have the synergistic effects of antagonism and induced resistance on plant disease.