中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2012年
12期
823-827
,共5页
李俊芝%王成辉%周福安%苗娜%古丽那尔·阿布拉江%张巍
李俊芝%王成輝%週福安%苗娜%古麗那爾·阿佈拉江%張巍
리준지%왕성휘%주복안%묘나%고려나이·아포랍강%장외
髓母细胞瘤%杂合子丢失%预后
髓母細胞瘤%雜閤子丟失%預後
수모세포류%잡합자주실%예후
Medulloblastoma%Loss of heterozygosity%Prognosis
目的 探讨髓母细胞瘤β-catenin蛋白的表达及10q杂合性缺失与临床病理特征的关系.方法 选择新疆医科大学第一附属医院2002至2011年间,经病理组织学诊断为不同类型的髓母细胞瘤合计50例,包括经典型髓母细胞瘤32例,促纤维增生型13例,结节型5例,采用免疫组织化学EnVision法对50例髓母细胞瘤石蜡切片β-catenin蛋白进行半定量分析,荧光原位杂交技术检测10q杂合性缺失,Kaplan-Meien和Cox回归分析β-catenin蛋白、10q杂合性缺失与临床病理特征及预后关系.结果 β-catenin蛋白在经典型髓母细胞瘤胞质中阳性率为53.1％ (17/32),促纤维增生型为4/13,广泛结节型为1/5；经典髓母细胞瘤10q杂合性缺失率为33.3％(8/24),促纤维增生型为2/11,结节型未见10q杂合性缺失(0/5)；三种类型间比较,差异均无统计学意义(P＞0.05).单因素分析β-catenin蛋白阳性表达者(P=0.022)、无10q杂合性缺失(P=0.020)、肿瘤切除范围(P＜0.01)、放疗(P =0.002)、化疗(P＜0.01)与髓母细胞瘤患者预后相关.结论 β-catenin蛋白阳性表达者、无10q杂合性缺失者预后较好.检测髓母细胞瘤染色体β-catenin蛋白表达和10q杂合性缺失,有助于预后判断.
目的 探討髓母細胞瘤β-catenin蛋白的錶達及10q雜閤性缺失與臨床病理特徵的關繫.方法 選擇新疆醫科大學第一附屬醫院2002至2011年間,經病理組織學診斷為不同類型的髓母細胞瘤閤計50例,包括經典型髓母細胞瘤32例,促纖維增生型13例,結節型5例,採用免疫組織化學EnVision法對50例髓母細胞瘤石蠟切片β-catenin蛋白進行半定量分析,熒光原位雜交技術檢測10q雜閤性缺失,Kaplan-Meien和Cox迴歸分析β-catenin蛋白、10q雜閤性缺失與臨床病理特徵及預後關繫.結果 β-catenin蛋白在經典型髓母細胞瘤胞質中暘性率為53.1％ (17/32),促纖維增生型為4/13,廣汎結節型為1/5；經典髓母細胞瘤10q雜閤性缺失率為33.3％(8/24),促纖維增生型為2/11,結節型未見10q雜閤性缺失(0/5)；三種類型間比較,差異均無統計學意義(P＞0.05).單因素分析β-catenin蛋白暘性錶達者(P=0.022)、無10q雜閤性缺失(P=0.020)、腫瘤切除範圍(P＜0.01)、放療(P =0.002)、化療(P＜0.01)與髓母細胞瘤患者預後相關.結論 β-catenin蛋白暘性錶達者、無10q雜閤性缺失者預後較好.檢測髓母細胞瘤染色體β-catenin蛋白錶達和10q雜閤性缺失,有助于預後判斷.
목적 탐토수모세포류β-catenin단백적표체급10q잡합성결실여림상병리특정적관계.방법 선택신강의과대학제일부속의원2002지2011년간,경병리조직학진단위불동류형적수모세포류합계50례,포괄경전형수모세포류32례,촉섬유증생형13례,결절형5례,채용면역조직화학EnVision법대50례수모세포류석사절편β-catenin단백진행반정량분석,형광원위잡교기술검측10q잡합성결실,Kaplan-Meien화Cox회귀분석β-catenin단백、10q잡합성결실여림상병리특정급예후관계.결과 β-catenin단백재경전형수모세포류포질중양성솔위53.1％ (17/32),촉섬유증생형위4/13,엄범결절형위1/5；경전수모세포류10q잡합성결실솔위33.3％(8/24),촉섬유증생형위2/11,결절형미견10q잡합성결실(0/5)；삼충류형간비교,차이균무통계학의의(P＞0.05).단인소분석β-catenin단백양성표체자(P=0.022)、무10q잡합성결실(P=0.020)、종류절제범위(P＜0.01)、방료(P =0.002)、화료(P＜0.01)여수모세포류환자예후상관.결론 β-catenin단백양성표체자、무10q잡합성결실자예후교호.검측수모세포류염색체β-catenin단백표체화10q잡합성결실,유조우예후판단.
Objective To study the expression of β-catenin protein and the status of loss of heterozygosity (LOH) on chromsome 10q in medulloblastoma,with clinical correlation.Methods Immunohistochemical study for β-catenin protein was carried out in 50 cases of medulloblastoma encountered in the First Affiliated Hospital of Xinjiang Medical University during the period from 2002 to 2011,including 32 cases of classic medulloblastoma,13 cases of desmoplastic medulloblastoma and 5 cases of medulloblastoma with extensive nodularity.The status of LOH on 10q was also detected by fluorescence in-situ hybridization.The clinicopathologic characteristics and prognostic parameters were studied by Kaplan-Meien and Cox analysis.Results The rates of expression of β-catenin protein in classic medulloblastoma,desmoplastic medulloblastoma and medulloblastoma with extensive nodularity were 53.1％ (17/32),4/13 and 1/5,respectively.The rate of LOH on 10q was 33.3％ (8/24) in classic medulloblastoma and 2/11 in desmoplastic medulloblastoma.There was no statistically significant difference between the two tumor types.Univariate analysis showed that the expression of β-catenin protein (P =0.022),lack of LOH on 10q (P =0.020),extensiveness of tumor resection (P ＜ 0.01),radiotherapy (P =0.002) and chemotherapy (P ＜ 0.01) represented important prognostic factors.Conclusions Medulloblastoma with expression of β-catenin protein and without LOH on 10q carries a better prognosis.Assessment of these parameters is helpful in evaluating prognosis and subsequent patient management.
