CAJ | 학술논문

目的：将pcDNA3.1（-）-ALR（hALR）和pcDNA3.1（-）分别转染人肝星形细胞（HSC），筛选在人肝星形细胞中存在表达差异的基因，进一步研究hALR的生物学功能及机制。方法以脂质体技术将pcDNA3.1（-）-ALR和pcDNA3.1（-）分别转染人肝星形细胞系，提取总mRNA，逆转录为cDNA，应用基因表达谱芯片方法分析差异表达的基因。结果在所筛选的4096个基因中，有2个基因表达水平显著上调，25个基因表达水平显著下调。结论hALR对于人肝星形细胞基因表达谱有显著影响，为进一步研究hALR的生物学功能和作用机制提供了线索。
목적：장pcDNA3.1（-）-ALR（hALR）화pcDNA3.1（-）분별전염인간성형세포（HSC），사선재인간성형세포중존재표체차이적기인，진일보연구hALR적생물학공능급궤제。방법이지질체기술장pcDNA3.1（-）-ALR화pcDNA3.1（-）분별전염인간성형세포계，제취총mRNA，역전록위cDNA，응용기인표체보심편방법분석차이표체적기인。결과재소사선적4096개기인중，유2개기인표체수평현저상조，25개기인표체수평현저하조。결론hALR대우인간성형세포기인표체보유현저영향，위진일보연구hALR적생물학공능화작용궤제제공료선색。
Objective To screening the differential gene expression in human hepatic stellate cells (HSC) treated with pcDNA3.1-ALR in order to investigate the biological functions and mechanism of human augmenter of liver regeneration (hALR). Methods HSC were transfected by pcDNA3.1 (-) and pcDNA3.1-ALR, respectively, using FuGENE6 transfection reagent. The total mRNA was isolated and reverse transcribed to synthesize the cDNA probes. Analyzed the differentially expressed genes by microarray gene expression profiling technique. Results Two genes were up-regulated and 25 genes were down-regulated among detected 4096 genes. Conclusions hALR has marked effect on gene expression proiflings of human HSC. These results provide new clues for studying the molecular biological function and mechanism of hALR.