中国肝脏病杂志（电子版）
中國肝髒病雜誌（電子版）
중국간장병잡지（전자판）
CHINESE JOURNAL OF LIVER DISEASES(ELECTRONIC VERSION)
2013年
4期
6-9
,共4页
纪世博%刘顺爱%全敏%冯胜虎%张梦然%张锦前%梁金秋%王琦%邢卉春%成军
紀世博%劉順愛%全敏%馮勝虎%張夢然%張錦前%樑金鞦%王琦%邢卉春%成軍
기세박%류순애%전민%풍성호%장몽연%장금전%량금추%왕기%형훼춘%성군
B7-H1%肝炎,乙型%癌,肝细胞%Survivin
B7-H1%肝炎,乙型%癌,肝細胞%Survivin
B7-H1%간염,을형%암,간세포%Survivin
B7-H1%Hepatitis B%Carcinoma,hepatocellular%Survivin
目的：探讨B7-H1（B7-homolog 1）在乙型肝炎病毒（HBV）感染导致的肝细胞癌（HCC）中的作用机制。方法通过慢病毒lentivirus-B7-H1转染人正常肝细胞系L02、人肝癌细胞系HepG2、HepG2.2.15后，应用MTT法观察细胞活性，流式细胞仪检测细胞的凋亡情况，Western blot技术检测蛋白B7-H1、Survivin蛋白的变化情况。结果抑制B7-H1表达水平后，通过MTT检测表明细胞系HepG2.2.15细胞活性比HepG2细胞、L02细胞活性降低，流式细胞仪检测凋亡增加，同时Western blot显示Survivin蛋白的表达减少。结论通过慢病毒转染抑制B7-H1的表达可以促进细胞系HepG2.2.15凋亡，B7-H1可能是通过调控Survivin的变化影响HBV感染的肿瘤细胞的增殖与凋亡。
目的：探討B7-H1（B7-homolog 1）在乙型肝炎病毒（HBV）感染導緻的肝細胞癌（HCC）中的作用機製。方法通過慢病毒lentivirus-B7-H1轉染人正常肝細胞繫L02、人肝癌細胞繫HepG2、HepG2.2.15後，應用MTT法觀察細胞活性，流式細胞儀檢測細胞的凋亡情況，Western blot技術檢測蛋白B7-H1、Survivin蛋白的變化情況。結果抑製B7-H1錶達水平後，通過MTT檢測錶明細胞繫HepG2.2.15細胞活性比HepG2細胞、L02細胞活性降低，流式細胞儀檢測凋亡增加，同時Western blot顯示Survivin蛋白的錶達減少。結論通過慢病毒轉染抑製B7-H1的錶達可以促進細胞繫HepG2.2.15凋亡，B7-H1可能是通過調控Survivin的變化影響HBV感染的腫瘤細胞的增殖與凋亡。
목적：탐토B7-H1（B7-homolog 1）재을형간염병독（HBV）감염도치적간세포암（HCC）중적작용궤제。방법통과만병독lentivirus-B7-H1전염인정상간세포계L02、인간암세포계HepG2、HepG2.2.15후，응용MTT법관찰세포활성，류식세포의검측세포적조망정황，Western blot기술검측단백B7-H1、Survivin단백적변화정황。결과억제B7-H1표체수평후，통과MTT검측표명세포계HepG2.2.15세포활성비HepG2세포、L02세포활성강저，류식세포의검측조망증가，동시Western blot현시Survivin단백적표체감소。결론통과만병독전염억제B7-H1적표체가이촉진세포계HepG2.2.15조망，B7-H1가능시통과조공Survivin적변화영향HBV감염적종류세포적증식여조망。
Objective To study the effect and mechanism of B7-H1 in the course that hepatitis B virus (HBV) caused hepatocelhlar carcinoma (HCC). Methods This study employed lentivirus mediated knockdown of B7-H1 to elucidate the oncogenic role of B7-H1 in human hepatocelhlar carcinoma HepG2 and HepG2.2.15 cell, MTT assay and lfow cytometry that B7-H1 knockdown induced decreased cell proliferation and increased apoptosis, Western blot used to test the expression of B7-H1 and Survivin. Results The transfected lentivirus-B7-H1 HepG2 and HepG2.2.15 cells induced decreased cell proliferation and increased apoptosis, and at the same time the expression of Survivin protein also decreased. Conclusions Knockdown of B7-H1 induced decreased cell proliferation and increased apoptosis of HepG2.2.15 cell, B7-H1 effected on proliferation and apoptosis of that HBV causes HCC which may have role in Survivin ways.
