中南大学学报（医学版）
中南大學學報（醫學版）
중남대학학보（의학판）
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2013年
12期
1228-1232
,共5页
ICR小鼠%博莱霉素%肺间质纤维化
ICR小鼠%博萊黴素%肺間質纖維化
ICR소서%박래매소%폐간질섬유화
Institute for Cancer Research mouse%bleomycin%pulmonary ifbrosis
目的：与单剂量气管内滴药模型比较，研究小剂量多次尾静脉注射博莱霉素(bleomycin，BLM)致小鼠肺纤维化的特点与差异。方法：40只雄性ICR(Institute for Cancer Research)小鼠随机分为模型组I、模型组II和两个对照组，每组10只。模型组I尾静脉注射10 mg/kg BLM均持续14 d；模型组II于实验第一天气管内注入5 mg/kg BLM，两对照组分别给与等量生理盐水，28 d后处死并收集肺泡灌洗液(bronchoalveolar lavage fluid，BALF)。检测BALF细胞总数及蛋白含量、肺系数、羟脯氨酸(hydroxyproline，HYP)，观察肺组织病理改变。结果：1)两种给药方法均能使肺组织发生明显的炎性和纤维化反应，两模型组BALF细胞总数及蛋白含量、肺系数、HYP含量、肺间质损伤指数较两对照组均显著增加(P﹤0.01)；2)模型组I病灶主要分布在胸膜下及血管周围，模型组II则主要分布在支气管和细支气管周围；3)模型组II病死率高于模型组I；4)模型组II BALF蛋白含量高于模型组I (P<0.05)；BALF细胞总数、肺系数、HYP含量、肺间质损伤指数两组间差异无统计学意义(P>0.05)。结论：小剂量多次尾静脉注射与气管内滴入BLM都能成功制备肺间质纤维化动物模型，但两者纤维化形成的部位存在着一定的差异，小剂量多次尾静脉给药肺间质纤维化模型更接近特发性肺间质纤维化。
目的：與單劑量氣管內滴藥模型比較，研究小劑量多次尾靜脈註射博萊黴素(bleomycin，BLM)緻小鼠肺纖維化的特點與差異。方法：40隻雄性ICR(Institute for Cancer Research)小鼠隨機分為模型組I、模型組II和兩箇對照組，每組10隻。模型組I尾靜脈註射10 mg/kg BLM均持續14 d；模型組II于實驗第一天氣管內註入5 mg/kg BLM，兩對照組分彆給與等量生理鹽水，28 d後處死併收集肺泡灌洗液(bronchoalveolar lavage fluid，BALF)。檢測BALF細胞總數及蛋白含量、肺繫數、羥脯氨痠(hydroxyproline，HYP)，觀察肺組織病理改變。結果：1)兩種給藥方法均能使肺組織髮生明顯的炎性和纖維化反應，兩模型組BALF細胞總數及蛋白含量、肺繫數、HYP含量、肺間質損傷指數較兩對照組均顯著增加(P﹤0.01)；2)模型組I病竈主要分佈在胸膜下及血管週圍，模型組II則主要分佈在支氣管和細支氣管週圍；3)模型組II病死率高于模型組I；4)模型組II BALF蛋白含量高于模型組I (P<0.05)；BALF細胞總數、肺繫數、HYP含量、肺間質損傷指數兩組間差異無統計學意義(P>0.05)。結論：小劑量多次尾靜脈註射與氣管內滴入BLM都能成功製備肺間質纖維化動物模型，但兩者纖維化形成的部位存在著一定的差異，小劑量多次尾靜脈給藥肺間質纖維化模型更接近特髮性肺間質纖維化。
목적：여단제량기관내적약모형비교，연구소제량다차미정맥주사박래매소(bleomycin，BLM)치소서폐섬유화적특점여차이。방법：40지웅성ICR(Institute for Cancer Research)소서수궤분위모형조I、모형조II화량개대조조，매조10지。모형조I미정맥주사10 mg/kg BLM균지속14 d；모형조II우실험제일천기관내주입5 mg/kg BLM，량대조조분별급여등량생리염수，28 d후처사병수집폐포관세액(bronchoalveolar lavage fluid，BALF)。검측BALF세포총수급단백함량、폐계수、간포안산(hydroxyproline，HYP)，관찰폐조직병리개변。결과：1)량충급약방법균능사폐조직발생명현적염성화섬유화반응，량모형조BALF세포총수급단백함량、폐계수、HYP함량、폐간질손상지수교량대조조균현저증가(P﹤0.01)；2)모형조I병조주요분포재흉막하급혈관주위，모형조II칙주요분포재지기관화세지기관주위；3)모형조II병사솔고우모형조I；4)모형조II BALF단백함량고우모형조I (P<0.05)；BALF세포총수、폐계수、HYP함량、폐간질손상지수량조간차이무통계학의의(P>0.05)。결론：소제량다차미정맥주사여기관내적입BLM도능성공제비폐간질섬유화동물모형，단량자섬유화형성적부위존재착일정적차이，소제량다차미정맥급약폐간질섬유화모형경접근특발성폐간질섬유화。
Objective:To determine the characteristics and differences in bleomycin-induced lung ifbrosis model by repeated low-dose intravenous injection and single dose intratracheal instillation of bleomycin. <br> Methods:Forty male ICR (Institute for Cancer Research) mice were randomly divided into a model group I, a model group II, and 2 control groups (10 mice in each group). In model group I, bleomycin was injected intravenously at 10 mg/(kg·d) for 14 consecutive days;and in model group II, bleomycin was instilled intratracheally at 5 mg/kg. The 2 control groups were given isotonic saline solution. At the 28th day, the mice were sacrificed and the bronchoalveolar lavage lfuid (BALF) was collected. The total cells and proteins in the BALF, pulmonary coeffcient, and hydroxyproline (HYP) content were determined. The pathological changes were observed by the eosin staining and Masson's trichrome staining. <br> Results:1) Both intravenous injection and intratracheal instillation of bleomycin resulted in severe and extensive inlfammation and ifbrosis in the lungs. The total cells and proteins in the BALF, HYP content, pulmonary coeffcient and the pathological score of pulmonary ifbrosis were all signiifcantly increased in the 2 model groups (P<0.01). 2) Fibrosis was mainly under the pleura or around the vessel in model group I, and it was located near the bronehia and bronchioles in model group II. 3) The death rate was higher in the model group II than that in the model group I. 4) Proteins in the BALF were significantly higher in model group II than that in model group I (P<0.05). There was no difference in the total cells in the BALF, the pulmonary coefficient, the HYP content, and the pathological score of pulmonary ifbrosis between the 2 groups (P>0.05). <br> Conclusion:The pulmonary fibrosis model can be successfully established by intravenous injection or intratracheal instillation of bleomycin, but the sites of pulmonary ifbrosis are different. The histological changes caused by the repeated low-dose intravenous injection of bleomycin is more similar to idiopathic pulmonary ifbrosis than that by the single dose intratracheal instillation.
