CAJ | 학술논문

目的：通过检测新生隐球菌感染小鼠巨噬细胞相关细胞因子的表达水平，探讨其在感染小鼠疾病病程的作用。方法应用单侧小鼠鼻孔接种感染新生隐球菌建立小鼠吸入感染隐球菌模型，在感染后第1、4、7、11、14、18、21天，PAS 染色观察小鼠肺组织病理变化，并通过 RT-PCR 检测相应时间点小鼠巨噬细胞内相关细胞因子（IL-6、IL-8、TGF-β、TNF-α）的表达。结果小鼠吸入感染隐球菌后，PAS 染色发现第4天肺内散在分布隐球菌，第7天可见肉芽肿形成，第11天大量炎性细胞浸润，第14天见肉芽肿内大量隐球菌，第18天隐球菌分布至全肺，第21天肺组织大量坏死；RT-PCR 结果显示 TGF-β和 IL-6的表达在感染后14天达到最高值，然后逐渐降低，其中 TGF-β升高幅度更为明显。结论在新生隐球菌感染小鼠中，TGF-β参与了机体的抗真菌免疫，在调节炎症反应方面有重要作用。
목적：통과검측신생은구균감염소서거서세포상관세포인자적표체수평，탐토기재감염소서질병병정적작용。방법응용단측소서비공접충감염신생은구균건립소서흡입감염은구균모형，재감염후제1、4、7、11、14、18、21천，PAS 염색관찰소서폐조직병리변화，병통과 RT-PCR 검측상응시간점소서거서세포내상관세포인자（IL-6、IL-8、TGF-β、TNF-α）적표체。결과소서흡입감염은구균후，PAS 염색발현제4천폐내산재분포은구균，제7천가견육아종형성，제11천대량염성세포침윤，제14천견육아종내대량은구균，제18천은구균분포지전폐，제21천폐조직대량배사；RT-PCR 결과현시 TGF-β화 IL-6적표체재감염후14천체도최고치，연후축점강저，기중 TGF-β승고폭도경위명현。결론재신생은구균감염소서중，TGF-β삼여료궤체적항진균면역，재조절염증반응방면유중요작용。
Objective We detected the expression of macrophage-derived cytokines in macrophage of Cryptococcus neoformans infection mouse model to explore the role of those cytokines in pathogenesis of infection mouse. Methods A mouse model of Cryptococcus neoformans infection was established by inhalation of Cryptococcus neoformans through one nostril. On day 1,4,7, 11,14,18,21 after inoculation,the infected lung were observed by histopathological analysis. Macrophage-derived cytokines(IL-6、IL-8、TGF-β、TNF-α)were detected by RT-PCR. Results Histopathological examination showed few Cryptococcus neoformans dis-tributed in lung on 4 days and granuloma formation on 7 days after infection. There were significant inflammatory cellular infiltration in the lung tissue on 11 days and a large number of Cryptococcus neoformans in granuloma on 14 days after infection. At 18 and 21 days,diffuse distribution of Cryptococcus neoformans in the lung and the lung necrosis were observed. The mRNA expression of TGF-βand IL-6 reached to the peak at 14 days,then decreased gradually. Conclusion TGF-βwas involved in regulating the anti-fungal immunity in Cryptococcus neoformans infection mouse.