CAJ | 학술논문

目的:观察舒血宁对高氧暴露早产鼠视网膜缺氧诱导因子-1α(hypoxia inducible factors-1α,HIF-1α)和血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响.方法:3日龄早产大鼠36只随机分为:高氧组、干预组、空气组,免疫组织化学法检测各组早产鼠视网膜HIF-1和VEGF表达水平.结果:空气组HIF-1α、VEGF均呈低表达,未见或罕见突破内界膜的内皮细胞核;与空气组相比,高氧组突破内界膜的内皮细胞核明显增多,HIF-1α及VEGF表达的表达均显著增加,(P分别<0.001,0.001,0.01),与高氧组相比,干预组突破内界膜的内皮细胞核明显减少,HIF-1α及VEGF的表达均显著下降(P分别<0.05,0.001,0.05);视网膜HIF-1α的表达与VEGF的表达、视网膜HIF-1α的表达与突破视网膜内界膜内皮细胞核数、VEGF的表达与突破视网膜内界膜内皮细胞核数均呈正相关(r分别为0.55,0.65,0.46,P分别<0.01,0.01,0.05).结论:高氧暴露早产鼠视网膜内可观察到VEGF和HIF-1α的高表达,舒血宁使高氧暴露早产鼠视网膜HIF-1和VEGF的表达明显下降,突破视网膜内界膜的内皮细胞核数量明显减少,减少新生血管的形成,对高氧性视网膜病有一定的保护作用.
목적:관찰서혈저대고양폭로조산서시망막결양유도인자-1α(hypoxia inducible factors-1α,HIF-1α)화혈관내피생장인자(vascular endothelial growth factor,VEGF)표체적영향.방법:3일령조산대서36지수궤분위:고양조、간예조、공기조,면역조직화학법검측각조조산서시망막HIF-1화VEGF표체수평.결과:공기조HIF-1α、VEGF균정저표체,미견혹한견돌파내계막적내피세포핵;여공기조상비,고양조돌파내계막적내피세포핵명현증다,HIF-1α급VEGF표체적표체균현저증가,(P분별<0.001,0.001,0.01),여고양조상비,간예조돌파내계막적내피세포핵명현감소,HIF-1α급VEGF적표체균현저하강(P분별<0.05,0.001,0.05);시망막HIF-1α적표체여VEGF적표체、시망막HIF-1α적표체여돌파시망막내계막내피세포핵수、VEGF적표체여돌파시망막내계막내피세포핵수균정정상관(r분별위0.55,0.65,0.46,P분별<0.01,0.01,0.05).결론:고양폭로조산서시망막내가관찰도VEGF화HIF-1α적고표체,서혈저사고양폭로조산서시망막HIF-1화VEGF적표체명현하강,돌파시망막내계막적내피세포핵수량명현감소,감소신생혈관적형성,대고양성시망막병유일정적보호작용.